Xanthoria parietina polysaccharides : isolation, characterization and influence on murine macrophages under in vitro conditions

In this study, polysaccharides from Xanthoria parietina, a wide spread lichenfrom central Europe, were isolated, purified and analyzed concerning thestructural features, in combination with functional investigation of the influenceon ,?1urine macrophage cell line RAW264.7 in vitro.Defatted dried lic...

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Main Author: Zalilawati Mat Rashid (Author)
Format: Thesis Book
Language:English
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008 151019s2015 my eng
040 |a UniSZA 
050 0 0 |a  SB431 
090 0 0 |a SB431   |b .Z35 2015 
100 1 |a Zalilawati Mat Rashid   |e author  
245 0 0 |a Xanthoria parietina polysaccharides :   |b isolation, characterization and influence on murine macrophages under in vitro conditions   |c Zalilawati Mat Rashid. 
264 0 |c 2015. 
300 |a 244 leaves:   |b ill. (some col.);   |c 20 cm. 
336 |a text  |2 rdacontent 
337 |a unmediated  |2 rdamedia 
338 |a volume  |2 rdacarrier 
502 |a Thesis (Degree of Doctor of Philosophy) - Universitat Munster, 2015 
504 |a Includes bibliographical references (leaves 226-244) 
505 0 |a 1. Introduction -- 2. Results -- 3. Discussion -- 4. Materials and methods 
520 |a In this study, polysaccharides from Xanthoria parietina, a wide spread lichenfrom central Europe, were isolated, purified and analyzed concerning thestructural features, in combination with functional investigation of the influenceon ,?1urine macrophage cell line RAW264.7 in vitro.Defatted dried lichen (140 g, WT) was submitted to consecutive cold water (4°C,12 h), freezing (-20°C) and thawing (room temperature), hot water (100°C) aswell as alkaline (0.5: M aq. NaOH) extractions (each in triplicate). Only hotaqueous extract, XH (2.8%, W/WT) was further fractionated by ion exchangechromatography to yield mainly H-1 (0.7%) and H-2 (0.4%) (W/WT) fractions.Chromatographic purification of the fractions by gel permeationchromatography yielded polysaccharides H-1-3 (0.39%) and H-2-1 (0.07%)(W/WT) with molecular weights Mwof 13.7 and 525 kDa respectively, prior toethanol precipitation, dialysis and lyophilisation.Monomers composition analysis by HPAEC-PADand GC-MSrevealed that H-1-3consisted mainly of glucose (> 98%), while H-2-1 was composed of man nose(74.3%), galactose (14.1%), glucose (9.8%) and rhamnose (1.8%) as well astrace of arabinose. O-/L-configuration of diastereomers analyzed by capillaryzone elactrophoresis (CZE) as well as the anomeric configuration by lH- and 13CNMRindicated the presence «-0- glucose in H-1-3 and c-D-manncse, «-0-galactose, u-D-glucose and c-t-Rhap in H-2-1. Subsequently, linkage sequenceswere determined by GC-MS analysis of partially methylated alditol acetates,nano-ESI QTOF-MS/MS analysis of oligosaccharides and 2D-NMR analysis ofnative polysaccharides and oligosaccharides. In nano-ESI-Q- TOF MS/MSanalysis, the use of silver adducts in positive-ion mode settings was establishedand found to be capable of rising A- and X-type fragment ions generated fromcross-ring cleavages, together with the rise of 8- and Y-type ions from theglycosidic bonds cleavages of the oligosaccharides prepared from H-1-3 but notH-2-1. For H-2-1, the use of chloride adduct in negative-ion mode settings wasbetter capable in rising similar type of fragment ions. 
610 2 0 |a Universitat Munster --   |x Dissertations  
650 0 |a Foliage plants --   |x Identification  
650 0 |a Foliage plants  
710 2 |a Universitat Munster  
999 |a 1000165043  |b Thesis  |c Reference  |e Tembila Thesis Collection