Molecular characterization and association of angiotensin converting enzyme (ACE) and peroxisome proliferator activated receptor V2 (PPARV2) genes in Malay obese attributes
Insel1ionldeletion (I/O) polymorphism of ACE gene and Pro 12Ala polymorphism in the peroxisome proliferator activated receptor Y2 (PPAR Y2) gene are among the identified candidate genes for obesity susceptibility. This study aimed to identify the genotypic and allelic frequencies of ACE gene 110 and...
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| Format: | Thesis Book |
| Language: | English |
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| Summary: | Insel1ionldeletion (I/O) polymorphism of ACE gene and Pro 12Ala polymorphism in the peroxisome proliferator activated receptor Y2 (PPAR Y2) gene are among the identified candidate genes for obesity susceptibility. This study aimed to identify the genotypic and allelic frequencies of ACE gene 110 and PPARY2 gene Prol2Ala polymorphisms and their associations with anthropometric parameters, lipid profiles and the susceptibility for obesity in Malay adult subjects. In this cross-sectional, comparative study, a total of 217 subjects (94 obese and 123 non-obese as controls) were included. Anthropometric parameters and lipid profiles were assessed. The lID and Prol2Ala variants were identified by allele-specific PCR. Genotypic and allelic distributions were analysed by Chi-square or Fisher's Exact test. A one-way ANOVA, independent sample t-test, Kruskal-Wallis or Mann-Whitney tests were used to compare the variables. A binary logistic regression was used to identify the significant risk factors for obesity. The ACE gene II, 10 and DO genotypes in all subjects were 51.6%, 39.2% and 9.2%, respectively. The frequencies of the I and 0 allele were 71.2% and 28.8%, respectively. The II, 10 and DO genotypic frequencies were similar in obese and non-obese groups (47.9% / 42.7% I 9.6% vs. 54.5% / 36.(i% I 8.9% respectively, P=0.620). Also, the 110 allelic frequencies were similar (61}.O% / 31.(1);0 vs, n.O% / 27.0% respectively, P=0.41 0). Stratification analysis based on gender did not show any difference between the groups, Anthropometric parameters and lipid profiles did not differ significantly between the ACE genotypes in both groups. The PPARY2 gene Pro12Pro, Prol2Ala and Alal2Ala genotypes were 93.5%,6.5% and 0.0%, respectively; the frequency of Pro 12 and Ala 12 allele was 96.8% and 32.0%, respectively. The Pro 12Pro, Pro 12Ala and Ala 12Ala genotypic frequencies were not similar between obese and non-obese groups (88.3% / 11.7% / 0.0% vs. 97.6% / 2.4% / 0.0% respectively, P=0.006). Additionally, the Ala 12 allele tended to be more frequent in obese than in the non-obese (5.9% vs. 1.1 %, P=0.007). Female obese had significantly higher Pro 12Ala genotype (18.0% vs. 2.2%, P=0.002) and Ala 12 allele (9.0% vs. 1.0%, P=0.002) than their female counterparts. There is a trend indicating the effect of Pro 12Ala polymorphism on body fat percentage and blood lipids in obese subjects. Analysis from univariate logistic regression showed that female gender, age and a high level of triglycerides, LDL-cholesterol and a low level of HDL-cholesterol and Ala 12 allele (OR 5.30, 95% CI= 1.44 - 19.59; P=O.O 12) were the risk factor for obesity. However, ACE gene 110 polymorphism cannot be regarded as the risk factor for obesity. In multivariate logistic regression, age, a higher level of triglycerides and LDL-cholesterol and Ala 12 allele (adjusted OR 5.46, 95% CI= 1.27 - 23.40; P=0.022) were regarded as independent risk factor for obesity. In conclusion, the Pro 12Ala polymorphi m in PPARY2 gene predisposes to obesity in Malay subjects and Alal2 allele, especially in obese subjects, could predict alterations in adipocyte and lipid metabolism among them. Besides aging, the PPARY2 gene Alal2 allele and a higher level of triglycerides and LDL-cholesterol were significantly associated with increased risk for obesity in this ethnicity. |
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| Physical Description: | xxiv, 241 leaves: illustration (some color); 30 cm. |
| Bibliography: | Includes bibliographical references (p. 190-215) |