Standardization, antioxidant activities and calcium oxalate crystals growth inhibition study of Melastoma malabathricum linn
Melastima malabathricum L., locally known as 'senduduk' belongs to the family Melastomataceae. The first part of the study was to standardize M. malabathricum samples based on simple quality control methods as well as FTIR-metabolite fingerprinting. The quality control of M. malabathricum...
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| Format: | Thesis Book |
| Language: | English |
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| Summary: | Melastima malabathricum L., locally known as 'senduduk' belongs to the family Melastomataceae. The first part of the study was to standardize M. malabathricum samples based on simple quality control methods as well as FTIR-metabolite fingerprinting. The quality control of M. malabathricum involved sampling the herb from different locations based on established morphological characteristics. The quality of samples were further tested by gravimetric analysis and phytochemical screening to determine yield percentage and presence of secondary metabolites for different parts of the plants. The results showed that samples were comparable to those described in herbal monographs. The FTIR-metabolite fingerprinting was then used to classify the samples according to locations. The powdered leaf samples were analysed by ATR-FTIR in the infrared region of 400-4000 cm-1. The data of 400-2000 cm-1 was analysed with multivariate techniques using principal component analysis (PCA) and linear discriminant analysis (LDA). The results of LDA using six principal components from PCA showed good classification with 78.6% of the cross-validated samples were correctly predicted. The second part of the study used extracts from four different parts of M. malabathricum for assessing the total phenolic content (TPC); total flavonoid content (TFC); antioxidant potential by DPPH free radical scavenging growth inhibition. The results showed that the flower extract was found to have highest phenolic content, DPPH free radicals scavenging activities as well as highest FRAP, while stem had the lowest values respectively. However, the leaf of M. malabathricum demonstrated the highest flavonoid content as well as crystal growth inhibition activity compared to other parts of plant. In the third part of the study, the lead extract was further fractionated by column chromatography. The fractions were studied for TFC as well as crystal growth inhibition and Spearman correlation showed that there was a good correlation between TFC and antilithiasis activity with the F1 fraction having highest activity. The activity of the F1 fraction was confirmed by calcium oxalate aggregation assay and microscopic examination. The higher content of flavonoids and calcium oxalate antilithiasis activity of the leaves suggest further studies for development of newer effective drugs from this herb. |
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| Physical Description: | xv, 95 leaves: ill. (some col.); 30 cm. |
| Bibliography: | Includes bibliographical references (leaves 80-90) |