Antioxidant activity and in-vitro antiproliferative effect of various solvent extracts of ficus carica fruit on breast cancer cell line (MCF-7)

Breast cancer is one of the commonest cancers with various biochemical therapeutic options but most of them are toxic to the body. Plants have been recognized as alternative sources of remedy for various ailments as they contain many phytochemical substances. This study was conducted to extract drie...

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Main Author: Rufa'i Umar Zubair (Author)
Format: Thesis Book
Language:English
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LEADER 04474cam a2200385 7i4500
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005 20201224090000.0
008 170110s2015 my eng
040 |a UniSZA 
050 0 0 |a TX553.A73 
060 1 0 |a WP 870  |b R922a 2015 
090 0 0 |a TX553.A73   |b R84 2015 
100 0 |a Rufa'i Umar Zubair   |e author  
245 1 0 |a Antioxidant activity and in-vitro antiproliferative effect of various solvent extracts of ficus carica fruit on breast cancer cell line (MCF-7)   |c Rufa'i Umar Zubair. 
264 0 |c 2015. 
300 |a xviii, 100 leaves:   |b ill. (some col.);   |c 30 cm. 
336 |a text  |2 rdacontent 
337 |a unmediated  |2 rdamedia 
338 |a volume  |2 rdacarrier 
502 |a Thesis (Degree of Master of Science) - Universiti Sultan Zainal Abidin, 2015 
504 |a Includes bibliographical references (p. 69-86) 
505 0 |a 1. Introduction -- 2. Literature review -- 3. Material and method -- 4.Results and discussion -- 5. Conclusion and recommendations 
520 |a Breast cancer is one of the commonest cancers with various biochemical therapeutic options but most of them are toxic to the body. Plants have been recognized as alternative sources of remedy for various ailments as they contain many phytochemical substances. This study was conducted to extract dried Ficus carica fruit using solvents of different polarity. The study aimed to determine the total phenolic contents, total flavonoid contents, antioxidant activity of all the extracts as well as to investigate their antiproliferative activity on breast cancer cell line (MCF-7) with mouse epithelial cell line (3T3) as a control. The Ficus carica fruit was extracted with three solvents i.e. ethanol (polar), ethyl acetate (semi polar) and hexane (non-polar). Total Phenolic Content (TPC) from all the extracts was estimated using Folin-Ciocalteu's reagent, while Total Flavonoid Content (TFC) was estimated using A1-Cl colorimetric method and both the results were compared to the standard curves accordingly. Determination of antioxidant was demonstrated by 2,2-diphenylpicryUlydrazyl (DPPH) scavenging assay while the antiproliferative activity of the extracts was determined using (3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide) ( MTT) assay. The correlation between the total phenolic, total Flavonoid and antioxidant activity as well as correlation between anti proliferative activity and antioxidant activity were also determined. The highest percentage of extract yield was obtained from ethanol solvent (18.72 %) compared to ethyl acetate (0.57 %) and hexane (0.48%) solvents. However the highest TPC (90.34±0.006 mg GAE/g extracts) and Total Flavonoid Contents (219±0.020 mg ofQEI g extracts) were obtained from ethyl acetate extracts followed by ethanol extract. The lowest TPC and TFC content was obtained from hexane extract. High antioxidant activity was demonstrated by ethyl acetate extract (ICso=580 ug/ml.), whereas ethanol and hexane extracts exhibit low antioxidant activity. A significant antiproliferative effect on breast cancer cell (MCF-7) was also demonstrated by ethyl acetate extract but not the ethanol and hexane extracts. Interestingly, no significant antiproliferative activity on mouse epithelial cell line was demonstrated by ethyl acetate extract. There was a positive correlation between TPC, TFC and antioxidant activity (r=.866, P<O.Ol). A negative correlation was also seen between the antioxidant activity and proliferative activity (r=.687 P<O.Ol). In conclusion the ethanol (polar) solvent showed the highest percentage extract yield compared to the ethyl acetate and hexane. However the highest TPC and TFC were obtained from ethyl acetate (semi polar) extract and this is positively correlated with its high antioxidant activity and antiproliferative activity against breast cancer cell (MCF-7). Therefore, these studies provide promising results on the chemotherapeutic potential of Ficus carica fruit extracts. 
610 2 0 |a Universiti Sultan Zainal Abidin --   |x Dissertations  
610 2 0 |a Universiti Sultan Zainal Abidin --   |x Faculty of Medicine  
650 0 |a Fig  
650 0 |a Fruit  
650 0 |a Breast Neoplasms  
650 0 |a Ficus (Plants)  
650 0 |a Antioxidants  
650 0 |a Cell Line  
650 0 |a In Vitro Techniques  
655 0 |a Dissertations, Academic 
710 2 |a Universiti Sultan Zainal Abidin  
999 |a 1000167580  |b Thesis  |c Reference  |e Medical Thesis Collection