Effects of apis dorsata honey on development of morphine tolerance in rats : behavioral and oxidative stress study

Repeated morphine administration can cause tolerance, in which the effect of the drug reduces and causes the need to increase the dose. Previous studies demonstrated the involvement of morphine tolerance with oxidative stress. Production of nitric oxide synthase (NOS) was proven to cause morphine to...

Full description

Saved in:
Bibliographic Details
Main Author: Nur Husna Zakaria (Author)
Format: Thesis Book
Language:English
Subjects:
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Repeated morphine administration can cause tolerance, in which the effect of the drug reduces and causes the need to increase the dose. Previous studies demonstrated the involvement of morphine tolerance with oxidative stress. Production of nitric oxide synthase (NOS) was proven to cause morphine tolerance. The increase of free radicals due to tolerance led to decrease in glutathione (GSH) and antioxidant enzymes, indicating elevated oxidative stress. Honey, a supersaturated solution produced by honeybees possesses rich bioactive compounds, antioxidant, and analgesic effect. Therefore, this study was carried out to determine the effect of A. dorsata honey on the development of morphine tolerance, total glutathione (GSH), malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) level in the brain of morphine-tolerant rats. Male Sprague Dawley rats were treated into five different groups (n=8) namely saline, morphine and morphine with honey (0.5, 1.5 & 2.5 g/kg) for 14 days. Assessment of morphine tolerance was carried out using hotplate test. On day 15, the animals were euthanised and the thalamus, spinal cord, and hippocampus were homogenized for the assessment of total GSH. iNOS, and MDA (oxidative stress biomarkers) using ELISA kits. A. dorsata honey significantly prevented morphine tolerance to analgesic in hotplate test on Day 14 (p<O.OS). The brain regions biochemical assessment showed that A. dorsata honey prevented GSH depletion compared to morphine control group. A. dorsata honey co-treatment also produced lower MDA compared to morphine control group with 2.5g/kg served as the significant dose. iNOS increased caused by morphine were lowered in A. dorsata honey co-treatment. A. dorsata honey prevented the development of morphine tolerance with attenuation of oxidative stress biomarkers. This study suggests the therapeutic role of A. dorsata honey in preventing morphine tolerance via inhibition of oxidative stress.
Physical Description:xx, 154 leaves: illustrations (some colour); 31 cm.
Bibliography:Includes bibliographical references (leaves 103-139)