Effects of apis dorsata honey on development of morphine tolerance in rats : behavioral and oxidative stress study
Repeated morphine administration can cause tolerance, in which the effect of the drug reduces and causes the need to increase the dose. Previous studies demonstrated the involvement of morphine tolerance with oxidative stress. Production of nitric oxide synthase (NOS) was proven to cause morphine to...
Saved in:
| 主要作者: | |
|---|---|
| 格式: | Thesis 图书 |
| 语言: | English |
| 主题: | |
| 标签: |
添加标签
没有标签, 成为第一个标记此记录!
|
| 总结: | Repeated morphine administration can cause tolerance, in which the effect of the drug reduces and causes the need to increase the dose. Previous studies demonstrated the involvement of morphine tolerance with oxidative stress. Production of nitric oxide synthase (NOS) was proven to cause morphine tolerance. The increase of free radicals due to tolerance led to decrease in glutathione (GSH) and antioxidant enzymes, indicating elevated oxidative stress. Honey, a supersaturated solution produced by honeybees possesses rich bioactive compounds, antioxidant, and analgesic effect. Therefore, this study was carried out to determine the effect of A. dorsata honey on the development of morphine tolerance, total glutathione (GSH), malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) level in the brain of morphine-tolerant rats. Male Sprague Dawley rats were treated into five different groups (n=8) namely saline, morphine and morphine with honey (0.5, 1.5 & 2.5 g/kg) for 14 days. Assessment of morphine tolerance was carried out using hotplate test. On day 15, the animals were euthanised and the thalamus, spinal cord, and hippocampus were homogenized for the assessment of total GSH. iNOS, and MDA (oxidative stress biomarkers) using ELISA kits. A. dorsata honey significantly prevented morphine tolerance to analgesic in hotplate test on Day 14 (p<O.OS). The brain regions biochemical assessment showed that A. dorsata honey prevented GSH depletion compared to morphine control group. A. dorsata honey co-treatment also produced lower MDA compared to morphine control group with 2.5g/kg served as the significant dose. iNOS increased caused by morphine were lowered in A. dorsata honey co-treatment. A. dorsata honey prevented the development of morphine tolerance with attenuation of oxidative stress biomarkers. This study suggests the therapeutic role of A. dorsata honey in preventing morphine tolerance via inhibition of oxidative stress. |
|---|---|
| 实物描述: | xx, 154 leaves: illustrations (some colour); 31 cm. |
| 参考书目: | Includes bibliographical references (leaves 103-139) |