Effects of honey on factors relating to cardiovascular disease based on in vitro and in vivo studies
Along history, honey has been used not only as a nutrient, but also as a medicine. Honey is rich in phenolic compounds, which act a natural antioxidants and are increasingly popular due to their potential in improving the human health. Thus, this study was designed to analyse some physico-chemical c...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Along history, honey has been used not only as a nutrient, but also as a medicine. Honey is rich in phenolic compounds, which act a natural antioxidants and are increasingly popular due to their potential in improving the human health. Thus, this study was designed to analyse some physico-chemical characteristics of two different types of honey, namely honey bee honey (Acacia) and stingless bee honey (Kelulut), to study the anti-inflammatory effects of polyphenols on the atherosclerosis modulation in human umbilical vein endothelial cells (HUVECs) and investigate the effects of these two honey for supplementation in subjects with cardiovascular risk factor. Ash, colour, water activity, viscosity, Brix, moisture, acidity, hidroxymethyl furfural (I MF), diastase activity and sugar analyses of the honey samples were assessed for chemical characterisation. Total phenolic compound, ferric reducing antioxidant capacity (FRAP) and 2, 2-diphenyl-l-picryhydrazyl (DPPH) free radical scavenging activity were IUnamllllre as antioxidant determinants. Acacia honey in this wed significantly higher content of colour (redness and yellowness), viscosity, Brix, sugar content, FRAP and total phenolic compared to those of Kelulut honey. The result also showed that Acacia honey had significantly lower content of water activity, moisture, acidity, HMF and diastase activity as compared to Kelulut honey. In this study on anti-inflammatory effects of polyphenols on the atherosclerosis modulation in HUVECs, secondary preventive effects of polyphenols (caffeic acid phenyl ester [CAPE], kaernpferol and quercetin) and their mixture were investigated via inhibition of ICAM-l and VCAM-1 expressions In (HUVECS) through enzyme-linked immunosorbent assay (ELISA) at 5, 10, 25, 50 and 100 /-lM with 24, 48 and 72 incubation period for a single compounds and ratios of 2: 1, 1: 1, 1:2 for the mixtures. Result showed that mixture of kaempferol and quercetin significantly enhance the anti-inflammatory effects by suppressing the ICAM-1 and VCAM-l expressions up to 132% and 101 %, respectively. Based on the intervention study in subjects with cardiovascular risk factors, it was revealed that 109/day Acacia honey supplementation significantly decreased blood glucose, total cholesterol, TO, LDL-c by 7.1 %, 7.6%, 16% and 5.4%, respectively. Meanwhile, 30 gram per day of Acacia honey supplementation significantly decreased blood glucose (7.1 %),total cholesterol (4.9%) and LDL-c (12.5%. n the rer hand, Kelulut honey supplementation only showed beneficial effects in higlln doses (30 g/day) by decreasing the total cholesterol (4.8%) significantly. Based on the lipid profile data, it can be concluded that Acacia honey intervention at 10 g/day exhibits better lipid lowering action. Thus, this study concluded that low dose of Acacia honey can be proposed as a nutritional dietary supplementation for those with cardiovascular disease risk modulation. |
|---|---|
| Physical Description: | xxv, 287 leaves; 30 cm. |
| Bibliography: | Includes bibliographical references (leaves 228-282 |