Comparative genomics alaysis and molecular characterization of community and hospital-associated methicillin-resistant Staphylococcus aureus
Published genomics data regarding CA-MRSA and HA-MRSA isolates from Malaysia are scarce. Therefore, the aim of this study was to characterise the Malaysian CA-MRSA and HA-MRSA isolates in depth using molecular typing, as well as whole-genome sequencing. CA-S aureus isolates were obtained from the na...
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| Format: | Thesis Book |
| Language: | English |
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| Summary: | Published genomics data regarding CA-MRSA and HA-MRSA isolates from Malaysia are scarce. Therefore, the aim of this study was to characterise the Malaysian CA-MRSA and HA-MRSA isolates in depth using molecular typing, as well as whole-genome sequencing. CA-S aureus isolates were obtained from the nasal swabs of undergraduate students whereas HA-S aureus isolates were archived samples obtained from hospital laboratories. Out of 168 S aureus nasal carriage isolates obtained, the occurrence of MRSA was 8.3% and IS% among undergraduate students of the agriculture biotechnology and health sciences programmes, respectively, with multidrug resistance were observed in IS% (26/168) of the isolates. Among the 146 hospital archived samples ofS aureus, 28% (41/146) were MRS A, out of which 63% (i.e. 26/41) were categorised as multidrug-resistant (MDR; resistant to three or more classes of antimicrobial compounds). The most predominant SCCmec type among both CA- and HA-MRSA was SCCmec-II1, with the highest occurrences observed among HA-MRSA (68%; 28/41) compared to CA-MRSA (41%; 9/22) isolates. Other SCCmec types that were found in CA-MRSA were SCCmec-IV (32%), SCCmec-I (23%), and SCCmec-II (4%), while in HA-MRSA were SCCmec-IV (22%), SCCmec- V (7%), and SCCmec-I (2%). spa type t037 was the most predominantly found among CA-MRSA (SO%) and HA-MRSA (61 %). Higher occurrence of the pvl gene (which encodes the pore-forming Panton-Valentine leucocidin) at 27% (11141) among HA-MRSA compared to CA-MRSA at 23% (S/22). Another virulence factor, the staphylococcal surface protein gene (sasX) , was more prevalent in HA-MRSA at 61 % (2S/41) compared with CA-MRSA (SO%; 11/22). Nine S. aureus isolates representing HA-MRSA (SAZ_1 SAZ_lO, AZ_16, SAZ_31, and KTIY21), hospital-associated methicillin-susceptible S. aureus (HA-MSSA, M314250), and CA-MRSA (ZS_Z30, ZS_Z37 and ZS_Z46) were subjected to wholegenome sequence analysis. Genomic content of all isolates were diverse with the presence of various mobile genetic elements (MGEs) such as insertional sequence (IS), transposoos, plasmids, genomic islands (vSAa and vSA |
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| Physical Description: | xx, 209 leaves; 26cm. |
| Bibliography: | Includes bibliographical references (leaves 150-188) |