Bioprotein production by phanarochaete chrysosporium using agro-waste as a cheap carbon source /
Bioprotein production is one of the most promising breakthroughs of biotechnological innovations. Due to its increasing demand, efficient strains, substrate and method must be used for high yield product. In this study, a potential and safe strain, Phanarochaete chrysosporium PC-13(PC2094) was used...
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| Format: | Thesis |
| Language: | English |
| Published: |
Gombak, Selangor :
Kulliyyah of Engineering, International Islamic University Malaysia,
2010
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Bioprotein production is one of the most promising breakthroughs of biotechnological innovations. Due to its increasing demand, efficient strains, substrate and method must be used for high yield product. In this study, a potential and safe strain, Phanarochaete chrysosporium PC-13(PC2094) was used to screen few agricultural wastes like honeydew, pineapple and durian skins as carbon source under liquid state bioconversion. Screening of the potential substrate was also done by conducting proximate analysis and experimental studies of the substrates as media. Optimized media was carried out in shake flask using two different statistical designs namely Plackett-Burman, to screen out the prominent media constituents and subsequently by using Face Centered Composite Design to optimize the selected constituents. Further development of optimized process conditions using the optimized media composition was done in bench-top bioreactor using half-fractional factorial design to obtain a maximum production. An amino acid analysis was carried out to determine the amino acid composition of the final product. Chemical oxygen demand and heavy metal test were done to check the quality of effluent based on Department of Environmental (DOE), Malaysia. From the screening studies, pineapple skin was found to be a potential carbon source due to its higher amount of carbohydrate and bioprotein production compared to other substrates. Based on the Plackett- Burman and Center Composite Design, the most significant parameters were pineapple skins soluble sugar (PSS) content, KH2PO4 and NH4H2PO4, which has optimal composition of 1.0%, 0.1% and 0.25%, respectively. A product of 514.2 g/kg bioprotein on the fifth day of fermentation with observed yield of 51.7 g/g-substrate and productivity of 121.93g/gsubstrate/ day was attained in a validation experiment. Process conditions experiment by half-fractional factorial design in 2 liter bench-top fermentor had identified agitation, aeration and temperature as the significant factors responsible for producing 772.17 g/kg-substrate of bioprotein at 150 rpm, 1.0 vvm and 37°C respectively. Compared to other results obtained in previous researches, the observed result are very significant as the percentage composition of weight for bioprotein for fungi should be in the range of 30% - 70% (w/w) for true proteins and 35% - 50% (w/w) for total nitrogen (protein plus nucleic acids). Eighteen essential amino acids were found in the bioprotein produced. COD was found to be removed by 91% throughout the fermentation period. Heavy metals like lead (Pb), chromium (Cr VI), cadmium (Cd) and zinc (Zn) were shown to have concentration below the permissible discharge limit set by DOE, Malaysia. This study may provide a better alternative in agricultural products by converting cheap carbon source to valuable and quality product bioprotein, which can be used as supplement and additive in the animal feed and food as well as in chemical and pharmaceutical industries. |
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| Item Description: | Abstract in English and Arabic. "A dissertation submitted in partial fulfilment of the requirements for the degree of Master of Science (Biotechnology Engineering)."--On t.p. |
| Physical Description: | xv, 127 leaves : ill. ; 30 cm. |
| Bibliography: | Includes bibliographical references (leaves 109-119) |