Antimicrobial effect of baccaurea angulata fruit extracts on human pathogenic microorganisms /
The research application for drugs and food supplements derived from plants extracts have increased in recent years. Plant extracts and their constituents are recognized to be safe, either because of their traditional use without any documented detrimental impact or because of dedicated toxicologica...
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Allied Health Sciences, International Islamic University Malaysia,
2015
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | The research application for drugs and food supplements derived from plants extracts have increased in recent years. Plant extracts and their constituents are recognized to be safe, either because of their traditional use without any documented detrimental impact or because of dedicated toxicological studies. On the other hand, human resistant pathogenic microorganisms increase worldwide mortality and morbidity. Due to emerging resistant and low susceptible strains to antibiotics, search for new alternative antimicrobials is raised significantly. Medicinal plants had been investigated and had been considered as potential sources of antimicrobial agents. Although hundreds of plant species have been tested for antimicrobial properties, the vast majority of medicinal plants have not been adequately evaluated. Thus, a systematic investigation was undertaken to screen for antibacterial activity from Baccaurea angulata (BA). The purpose of this study was to explore bacteriostatic and bactericidal effect of methanol, ethanol and aqueous extracts of three parts (whole fruit, fruit skin and berry) of BA fruit in vitro against tested pathogenic microorganisms. Total 9 extracts from three parts of BA fruit (whole fruit, fruit skin and berry) were tested against Gram positive (Staphylococcus aureus, Streptococcus pneumonia, Staphylococcus epidermidis, and Clostridium botulinum) Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella paratyphi A), and fungus (Candida albicans), using disk diffusion, agar well diffusion and microdilution methods. Results show that BA fruit extracts have potential antimicrobial properties against all tested microorganisms, it is various between three parts (whole fruit, fruit skin, and berry), three solvents (methanol, ethanol and aqueous), different method (agar well diffusion, disk diffusion and microdilution method) and different aforementioned pathogens. The highest inhibitory activity was observed in ethanol extract of fruit skin using agar well diffusion against Streptococcus pnumoniae (37.0±1.0 mm) at the concentration of 1,000.0 μg/mL. Among tested Gram negative bacteria K. pnumoniae was the most susceptible bacterium. The extracts showed minimum inhibitory and minimum bactericidal activity at the concentration of 7.8 and 15.6 μg.mL respectively using microdilution method. |
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| Physical Description: | xiv, 81 leaves : ill. ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 62-71) |