rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Isolation of low molecular mass antibacterial compound from supermeal worm Zophobas Morio fabricius
  name:
    name part: Johan Ariff bin Mohtar
    role:
      role term: creator
  name:
    name part: International Islamic University Malaysia
    name part: Department of Biotechnology Engineering
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    place:
      place term: Kuala Lumpur
    publisher: Kulliyyah of Engineering, International Islamic University Malaysia
    date issued: 2015
  language:
    language term: eng
  physical description:
    extent: xviii, 244 leaves : ill. ; 30cm.
  abstract: Lower molecular mass (< 1 kDa) insect antimicrobial compounds play a major role as the defensive armament against deadly microbial invasions. Their potent inhibitory effects display high potentials in many therapeutic applications and developments. The aim of this research is to explore the extraction, optimization of the process extraction conditions and partial purification of such compound from Zophobas morio supermealworms. Larvae were subjected to a solvent screening study incorporating acidified hydro-alcoholic extraction using water, methanol, ethanol, isopropanol and butanol to select the best solvent system based on the formation of large inhibition zone on bacteria in the susceptibility assay. Factors affecting the process extraction conditions including homogenizing time; solid-to-solvent ratio and temperature were statistically optimized using Response Surface Methodology (RSM) for maximal recovery. The antibacterial compound, collectively known as Zm-NI factor was partially purified by the bioassay-guided solid phase extraction on the reversed-phase and normal phase columns and was further analyzed by the analytical profiling. Isopropanol (90:9:1,v/v/v) was selected as the best solvent for Zm-NI factor recovery as its extract produced significantly (p < 0.05) larger inhibition zones on S. aureus (IMR: S822) (15.70 ± 0.33 mm), E. coli (IMR: E255) (14.53 ± 1.53 mm), K. pneumoniae (IMR: K36) (14.00 ± 1.00 mm) and P. aeruginosa (IMR: P167) (13.30 ± 0.58 mm) compared to other solvent extracts. S. aureus (IMR: S822) was the most susceptible towards the isopropanolic extract. Zm-NI factor exhibited a broad-spectrum antibacterial activity. It is a constitutive, non-viable, polar molecule with high resistance to trypsin, actively stable at high temperature and wide pH range. Its antibacterial activity is negatively affected by EDTA, SDS and β-mercaptoethanol. The optimization of the extraction process conditions by a Box-Behnken design (BBD) at a fixed isopropan…
  note: by Johan Ariff bin Mohtar
  note: Thesis (MSBTE)--International Islamic University Malaysia, 2015.
  note: Includes bibliographical references (leaves 185-224).
  subject:
    geographic code: a-my---
  classification: RS431.P37
  identifier: http://studentrepo.iium.edu.my/handle/123456789/4942
  record info:
    record content source: UIAM
    record creation date: 150910
    language of cataloging:
      language term: eng