rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Bioprospecting of mangrove actinomycetes and their antimicrobial potentials
  name:
    name part: Nurfathiah binti Abdul Malek
    role:
      role term: creator
  name:
    name part: International Islamic University Malaysia
    name part: Department of Biotechnology
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    place:
      place term: Kuantan, Pahang
    publisher: Kulliyyah of of Science, International Islamic University Malaysia
    date issued: 2016
  language:
    language term: eng
  physical description:
    extent: xx, 157 leaves : ill. ; 30cm.
  abstract: Actinomycetes, a group of Gram-positive bacteria within the order of Actinomycetales are widely distributed in nature. They are renowned for their unrivalled capacity to produce various bioactive metabolites, particularly antibiotics. Even though thousands of antimicrobial agents have been discovered from actinomycetes, these represent only a tiny portion of the entire repertoire. Exploration of poorly explored habitat offers a promising source of bioactive actinomycetes. Less attention has been given in understanding the diversity and biopotential of actinomycetes from mangrove ecosystem. Consequently, the present study was designed to isolate and screen antimicrobial producing actinomycetes from sediment of Kuantan mangrove. A total of 4850 isolates have been successfully enumerated from 7 study sites at Kuantan mangrove forest using various pretreatment procedures and selective isolation media. Wet heat pretreatment (60 ºC for 20 min) yielded the highest number of actinomycetes recovery. Moist heating at mild temperature inhibits the growth of unwanted contamination, while stimulates the colonization of actinomycetes. Actinomycetes from sediment of Kuantan mangrove forest favours isolation medium with high carbon-to-nitrogen ratio. The inorganic salt-starch (ISP4) agar medium displayed the best percentage of recovery (31.1 %), followed by actinomycete isolation (AIA, 24.1 %). A total of 140 representative isolates were selected for further evaluation based on an approach known as dereplication which allows rapid screening of large ambiguous actinomycetes collection. Determination on the antimicrobial activity of the isolates were conducted in a series of susceptibility tests against 5 test organisms. Preliminary screening revealed that 55.7 % of the total representative isolates possess antagonistic potential against at least one test organisms. The bioactive isolates were subjected to liquid fermentation and antimicrobial activity of their crude ethyl acetate ex…
  note: by Nurfathiah binti Abdul Malek
  note: Thesis (MSBTS)--International Islamic University Malaysia, 2016
  note: Includes bibliographical references (leaves 124-148).
  subject:
    geographic code: a-my---
  classification: QR82.A35
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/zzlS0wMAAAIzgT2PS114mdBX02vv3OJU20161109123231838
  record info:
    record content source: UIAM
    record creation date: 160908
    language of cataloging:
      language term: eng