rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Production and characterization of a thermostable and organic solvent tolerant lipase by isolated fungi using palm kernel cake
  name:
    name part: Riyadi, Fatimah Azizah
    role:
      role term: creator
  name:
    name part: International Islamic University Malaysia
    name part: Department of Biotechnology Engineering.
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    place:
      place term: Gombak, Selangor
    publisher: Kulliyyah of Engineering, International Islamic University Malaysia
    date issued: 2016
  language:
    language term: eng
  physical description:
    extent: xvii, 134 leaves : ill. ; 30cm.
  abstract: This research sought to explore the potential of palm kernel cake (PKC) to act as an oil and lipid rich environment for fungal growth, as well as a substrate to produce thermostable and organic solvent tolerant lipase through solid-state bioconversion. Four thermal and solvent tolerant fungi have successfully isolated with lipase producing capabilities. Among the four isolates, PKC12B2 displayed highest lipase activities and selected as the potential strain for the subsequent lipase production. Statistical analysis in the form of definitive screening design (DSD), one-factor-at-a-time (OFAT) and faced centered central composite design (FCCCD) were used to obtain optimum solid-state fermentation (SSF) medium compositions and process conditions for maximum lipase production. Through DSD as the initial screening step, peptone, (NH4)2SO4, olive oil, inoculum size, pH and moisture content were found to have the most significant contribution toward lipase production in PKC-based medium. Meanwhile, the classical OFAT studies revealed that olive oil, inoculum size and pH were associated with the high lipase production. Based on this, optimization of these three factors was carried out using FCCCD to develop a second-order regression model and a successful improvement in the lipase production was achieved. The optimum lipase production was 58.63 U/gds, which was attained within 72 hours fermentation time at 45 oC under the optimum conditions of 2 %v/w inoculum size, 2 %v/w olive oil, 0.6 %w/w peptone, 2 % ethanol as well as 70.0 % initial moisture content at pH of 10.0. PKC12B2 lipase was also subjected for biochemical characterizations in terms of activity and stability towards different pH, temperature, organic solvents and surfactants. The optimum pH for the enzyme was at pH 9.0 with good stability between pH 8.0 and 10.0, indicating its alkaline nature. The enzyme produced was also considered thermostable, possessing optimum temperature at 45 oC and thermal stability in …
  note: by Fatimah Azizah Riyadi
  note: Thesis (MSBTE)--International Islamic University Malaysia, 2016
  note: Includes bibliographical references (leaves 114-128).
  subject:
    geographic code: a-my---
  classification: TP248.65.E59
  identifier: http://studentrepo.iium.edu.my/handle/123456789/5145
  record info:
    record content source: UIAM
    record creation date: 161130
    language of cataloging:
      language term: eng