rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Hepatitis C virus infection among patients at HTAA
    sub title: genotypes distribution, RNA sequence variation, risk factors and comorbidities
  name:
    name part: Siti Nurul Fazlin binti Abdul Rahman
    role:
      role term: creator
  name:
    name part: International Islamic University Malaysia
    name part: Department of Basic Medical Sciences
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    place:
      place term: Kuantan, Pahang
    publisher: Kulliyyah of Medicine, International Islamic University Malaysia
    date issued: 2015
  language:
    language term: eng
  physical description:
    extent: xiv, 115 leaves : ill. ; 30cm.
  abstract: Hepatitis C virus (HCV) genotyping is very important for the clinical management of HCV-infected patients. HCV strains demonstrate a high degree of genetic heterogeneity. The 5' un-translated region (5'UTR) of HCV is the most highly conserved region of the virus genome, making this region ideal target for HCV RNA amplification-based detection assays. Additional target to determine the genotypes of HCV is the non-structural (NS) 5B region of the viral genome. The aim of this study was to determine HCV genotypes and identify sequence variances in common genotypes as well as their association with certain factors. Nucleotide sequence analysis of 5'UTR and NS5B region were performed to identify viral genotypes of 40 HCV infected patients attending Gastroenterology Clinic, HTAA Kuantan Pahang. Of the 40 samples, 39 (97.5%) were tested positive for HCV RNA. RNA-positive isolates were subjected to direct sequencing and the results were compared with reference sequences from NCBI homepage. Based on the analysis of both of the above regions, 31 (77.5%) isolates were successfully classified into their genotypes and subtypes; 3a (57.5%), 1a (12.5%), 3b (2.5%) and 1b (2.5%). No genotype 2, 4, 5 and 6 were found in this study. However, there was one mixed-genotype (3a/1a) HCV infection. Sequence variations were analyzed in both regions, NS5B region demonstrated greater genetic variability in its sequence than 5'UTR. No significant association was found between HCV genotypes with patient's demographic data, risk factors, alcohol consumption and co-infection with HBV or HIV. In conclusion, the use of the 5'UTR and NS5B-based assays proved to be a sensitive and reliable method for HCV RNA detection and genotyping. Genotype 3 followed by genotype 1 were the common HCV genotypes found in this study population. The base sequences of both genotypes varied at certain locations in both the 5'UTR and NS5B regions however NS5B region sequences displayed higher heterogeneity. Furthermore, n…
  note: by Siti Nurul Fazlin binti Abdul Rahman
  note: Thesis (MMDSC)--International Islamic University Malaysia, 2015.
  note: Includes bibliographical references (leave 97-107).
  subject:
    geographic code: a-my---
  subject:
    topic: Hepatitis C virus
  classification: RC848.H425
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/ZdOqWtNlygn8hJ5rPIb1aInZ0U0nNkXw20160406144832166
  record info:
    record content source: UIAM
    record creation date: 151210
    language of cataloging:
      language term: eng