Cinnamomum Burmannii's (Padang's cinnamon) essential oil and cinnamaldehyde antimicrobial activity, mode of action and synergistic effect /
Multiple drugs resistance organisms (MDRO) and antibiotics side effects are among the most worrisome issues in an effort to treat infectious diseases. Plant-based products had become the source of interest in pharmacological industry. Cinnamomum burmannii is one of the species in Cinnamomum genus an...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Science, Internatiional Islamic University Malaysia,
2014
|
| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Multiple drugs resistance organisms (MDRO) and antibiotics side effects are among the most worrisome issues in an effort to treat infectious diseases. Plant-based products had become the source of interest in pharmacological industry. Cinnamomum burmannii is one of the species in Cinnamomum genus and belongs to Lauraceae family. In traditional medicine, it was used to treat flatulence and nausea. The aims of the present study were to investigate the antimicrobial activity of essential oil and its major compound, cinnamaldehyde, as well as to examine their synergistic effect when combined with the current antibiotics. This study also aimed to study their mode of action on the cell membrane of selected microorganisms. Steam distillation was used for the extraction of the essential oil. The isolation of active compound from the essential oil was carried out using column chromatography and the characterization of the compound was analyzed using spectroscopic techniques. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) were determined against Staphylococcus aureus, Escherichia coli and Candida albicans. Synergistic effect was tested upon combination of the essential oil as well as cinnamaldehyde with antibiotics such as ampicillin, chloramphenicol and streptomycin against S. aureus and E. coli, while nystatin and amphotericin B was tested in combination against C. albicans using micro-dilution methods. There were four modes of action tested, which included time-killing, salt tolerance, crystal violet assay and leakage of cellular metabolites. All of the tests were done in triplicate. The MIC value of essential oil and cinnamaldehyde against all of tested microorganisms was found at concentration of0.33mg/mL while for their MBC/MFC value, 1 mg/mL was obtained against all microbial strains except for cinnamaldehyde against C. albicans (0.33 mg/mL). The Fractional Inhibitory Concentration (FIC) index upon combination was in the range of 1< FIC Index ≤2 which was evaluated as indifference effect. The antimicrobial action on the cell membrane was dose-dependent whereby a stronger action was observed by cinnamaldehyde at a concentration equal to 4×MIC (1.33 mg/ml) as compared to the essential oil. The present study was able to clarify the crucial role of cinnamaldehyde as a potent antimicrobial compound of the cinnamon essential oil and proven to act on the cell membrane of microorganisms tested particularly against C. albicans. |
|---|---|
| Physical Description: | xviii, 103 leaves : ill. ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 88-99). |