rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Isolation and microencapsulation of fucoxanthin for drug delivery system of human lung cancer (H1299) cell line
  name:
    name part: Noviendri, Dedi
    role:
      role term: creator
  name:
    name part: International Islamic University Malaysia
    name part: Kulliyyah of Education
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    place:
      place term: Kuala Lumpur
    publisher: Kulliyyah of Engineering, International Islamic University Malaysia
    date issued: 2014
  language:
    language term: eng
  physical description:
    extent: xxiii, 201 leaves : ill. ; 30cm.
  abstract: Fucoxanthin is the major xanthophyll in brown seaweed. This compound has anticancer activities but is poorly soluble in water, sensitive to light and temperature. The challenge in utilizing this carotenoid is normally associated with extraction, purification, protection, improvement of solubility and its application as anticancer activity. In this research work, fucoxanthin from four different species of Malaysian brown seaweeds, i.e. Sargassum binderi, S. duplicatum, Turbinaria turbinata and Padina australis was extracted and purified by solvent extraction. The purification was conducted by preparative high performance liquid chromatography (HPLC) double column with methanol and acetonitrile as the mobile phase at 450 nm. Fucoxanthin-loaded microsphere (F-LM) was fabricated using w/o/w double emulsion solvent evaporation method by microencapsulation (ME) technique. The effect of four surfactants (polivinylalcohol, PVA; Tween-20; Span-20 and sodium dodecyl sulphate, SDS), homogenization speed, concentration of poly(D, L-lactic-co-glycolic acid, PLGA) and surfactant (PVA) was investigated, and all of them influenced the particle size (PS) and morphology of F-LM. The properties of F-LM such as PS, morphology, thermal behavior, glass transition temperature (Tg), functional groups and its crystallinity were characterized. Subsequently, F-LM was optimized for in vitro testing on human lung cancer (H1299) cell line based on PS and encapsulation efficiency (EE) by ME technique using RSM. The optimum conditions to fabricate F-LM formulation were achieved by using a combination of 0.5% (w/v) PVA, 6.0% (w/v) PLGA and 200 µg/ml fucoxanthin composition. The optimum of PS and EE were found to be 9.18 µm and 33.09%, respectively. The morphology of F-LM produced were spherical, discrete and smooth surface with no pore. In vitro fucoxanthin release profile of F-LM was characterized by a typical biphasic behavior with burst release (38.3%) and predominantly controlled by Higuchi mod…
  note: by Dedi Noviendri
  note: Thesis (Ph.D)--International Islamic University Malaysia, 2014.
  note: Includes bibliographical references (leaves 159-193).
  subject:
    geographic code: a-my---
  classification: RC280.L8
  identifier: http://studentrepo.iium.edu.my/handle/123456789/4941
  record info:
    record content source: UIAM
    record creation date: 140912
    language of cataloging:
      language term: eng