rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Extraction and phytochemical profile of garlic metabolites and its use as alternative source of cycteine and glutathione
  name:
    name part: Nur Izzah binti Ismail
    role:
      role term: creator
  name:
    name part: International Islamic University Malaysia
    name part: Department of Biotechnology Engineering
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    place:
      place term: Kuala Lumpur
    publisher: Kulliyyah of Engineering, International Islamic University Malaysia
    date issued: 2014
  language:
    language term: eng
  physical description:
    extent: xviii, 102 leaves : ill. ; 30cm.
  abstract: Compounds that contain sulphur are known as thiols or sulfhydryl compounds. Cysteine and glutathione (GSH) which are sulphur-containing antioxidants are classified in this group. Apart being powerful antioxidants, they are widely used in food, pharmaceutical and cosmetic industries. Production of cysteine and GSH can be achieved through several methods such as chemical synthesis, enzymatic catalysis, microbial fermentation, and genetic/metabolic engineering. Although cysteine and GSH are abundantly present in plants but less was focused on extracting these compounds from plant sources. Therefore, this study intended to explore the potential of garlic as an alternative source for both compounds. Garlic was chosen based on the pungent smell produced by garlic which is correlated to high sulphur contents. Waterbased extraction assisted by sonication was used to obtain cysteine and GSH. This method was considered as it was simple, safe and cost effective for extractingthiols. Optimization of process conditions was performed using One Factor at a Time (OFAT) design. Cysteine and GSH were quantified as thiols/sulfhydryl compoundsusingEllman's reagent method. Global metabolites analysis approach using gas chromatography mass spectrometry (GCMS)and principal component analysis (PCA)was applied to study the effect of process conditions on the metabolites as a means to achieve optimal cysteine and GSH production. With respect to OFAT study, the optimum concentration of extracted thiols (0.1700 mM) was obtained at garlic concentration of 100 %w/v with 100 % amplitude of sonicator for 15s of extraction time and process temperature at 25°C. Based on GCMS analysis, it showed metabolites present in garlic which are amino acids and its derivatives, sugars, sugar alcohols, and organic acids. PCA successfully grouped garlic samples based on sample preparation and solvent extraction used, however it was unable to discriminate the garlic sample based on different process conditions sin…
  note: by Nur Izzah binti Ismail
  note: Thesis (MSBTE)--International Islamic University Malaysia, 2014.
  note: Includes bibliographical references (leaves 78-87).
  subject:
    geographic code: a-my---
  classification: QK495.A42
  identifier: http://studentrepo.iium.edu.my/handle/123456789/4811
  record info:
    record content source: UIAM
    record creation date: 140512
    language of cataloging:
      language term: eng