rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Antihyperglycaemic activities of xanthone rich extract of mangosteen (garcinia mangostana)
  name:
    name part: Tengku Muhamad Faris Syafiq bin Tengku Zakaria
    role:
      role term: creator
  name:
    name part: International Islamic University Malaysia
    name part: Department of Pharmaceutical Technology
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    place:
      place term: Kuantan
    publisher: Kulliyyah of Pharmacy, International Islamic University Malaysia
    date issued: 2015
  language:
    language term: eng
  physical description:
    extent: xviii, 159 leaves : ill. ; 30cm.
  abstract: Mangosteen (Garcinia mangostana Linn.) fruit pericarp has been used for centuries as a folk medicine. The study was conducted to evaluate the in vitro and in vivo antihyperglycaemic potential of G. mangostana extract (GME). The α-mangostin content in the extract was measured using HPLC and investigated for total phenolic and flavonoid contents. Antioxidant activities were measured by DPPH radical scavenging and reducing power assays whereas in vitro antidiabetic activities were evaluated by inhibition of α-glucosidase and α-amylase enzymes. Effects of GME on adipocyte cells were assessed through MTT assay, adipogenesis and glucose uptake measurements. In animal study, oral administration of GME1 (50 mg/kg), GME2 (100 mg/kg) and GME3 (200 mg/kg) to STZ-induced diabetic rats in single-dose (acute) and multiple-dose study (sub-acute) were examined. Serum biochemical parameters and histopathological alterations were evaluated and compared to standard hypoglycaemic drug, glibenclamide. The results showed that total phenolic and total flavonoid contents were 122.2±1.04 mg GAE/g and 72.8±1.75 mg QE/g of dry extract, respectively. DPPH radical scavenging activity and reducing power capacity reported with EC50 of 48.2 μg/ml and IC50 of 98.2 μg/ml, respectively. The enzymatic inhibition of α-glucosidase and α-amylase revealed higher percentage of inhibition comparable to acarbose with IC50 of 0.41 and 0.24 mg/ml, respectively. In cellular study, cytotoxicity assay reported that dose of less than 12.5 μg/ml does not affect cell viability. The differentiations of adipocytes were increased with higher GME concentration at 2.5 μg/ml, 5.0 μg/ml and 10.0 μg/ml. Glucose uptake measurements revealed a higher uptake of 2-deoxyglucose in GME2-treated cell (2243.3±232.3 cpm) as compared to GME1 (1864.0±146.3 cpm) and GME3 (1246.0±155.8 cpm). Oral administration of GME on diabetic rats indicated safe usage with absence of behavioural alterations, autonomic, neurological and toxic effects…
  note: by Tengku Muhamad Faris Syafiq bin Tengku Zakaria
  note: Thesis (MSPHT)--International Islamic University Malaysia, 2015.
  note: Includes bibliographical references (leaves 132-151).
  subject:
    geographic code: a-my---
  classification: RM666.M3245
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/sJhuYVigfpSpXTiSpkepfH7mrZ4g1mqt20150710111101093
  record info:
    record content source: UIAM
    record creation date: 150518
    language of cataloging:
      language term: eng