Effect of chronic organophosphate exposure on antioxidant enzyme activities, oxidized-LDL level and aortic microscopic changes in rats /

Organophosphates (OP) are hydrolysed by paraoxonase (PON1), an antioxidant enzyme which has been recognized to inhibit atherosclerosis event by deterring oxidative modification of low density lipoprotein (LDL). Low PON1 activities have been observed among individuals chronically exposed to OP while...

Full description

Saved in:
Bibliographic Details
Main Author: Zamzuria binti Mat Zainone
Format: Thesis
Language:English
Published: Kuantan : Kulliyyah of Medicine, International Islamic University Malaysia, 2015
Subjects:
Online Access:Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library.
Tags: Add Tag
No Tags, Be the first to tag this record!
LEADER 033150000a22002770004500
008 170511t2015 my a g m 000 0 eng d
040 |a UIAM  |b eng 
041 |a eng 
043 |a a-my--- 
050 |a RA1242.P56 
100 0 |a Zamzuria binti Mat Zainone 
245 1 |a Effect of chronic organophosphate exposure on antioxidant enzyme activities, oxidized-LDL level and aortic microscopic changes in rats /  |c by Zamzuria binti Mat Zainone 
260 |a Kuantan :  |b Kulliyyah of Medicine, International Islamic University Malaysia,  |c 2015 
300 |a xviii, 88 leaves :  |b ill. ;  |c 30cm. 
502 |a Thesis (MMDSC)--International Islamic University Malaysia, 2015. 
504 |a Includes bibliographical references (leaves 74-84). 
520 |a Organophosphates (OP) are hydrolysed by paraoxonase (PON1), an antioxidant enzyme which has been recognized to inhibit atherosclerosis event by deterring oxidative modification of low density lipoprotein (LDL). Low PON1 activities have been observed among individuals chronically exposed to OP while decreased activities were reported in individuals with higher risk of coronary artery disease (CAD). However, the relationship between chronic OP exposure, PON1 and the development of atherosclerosis has not yet been established. The aims of this study were to investigate the effects of chronic OP exposure on oxidative stress markers and to assess the aorta for atherosclerosis changes in animal model chronically and intermittently exposed to low dose OP. This study involved 20 male Sprague-Dawley rats, were divided into 3 groups; the first group did not receive any injection, while both Group 2 and Group 3 received subcutaneous injection of vehicle and injection of 18.0 mg/kg of chlorpyrifos (CPF) respectively every other day for 180 days. Blood were analysed for PON1 activities and oxidized LDL (ox-LDL), hearts were homogenized for superoxide dismutase (SOD) and catalase (CAT), and aorta were harvested and processed for microscopic examination. The PON1 activities, ox-LDL level and PON1:ox-LDL ratio were found to be significantly lower in OP exposed rats but the intracellular antioxidant enzyme activities were insignificant. Microscopically, the OP exposed rats showed early atherosclerosis changes with positive VCAM-1 expression. In conclusion, chronic intermittent low dose of OP CPF induced the development of early stage of atherosclerosis and this could be due to the inability of the PON1 to hydrolyse ox-LDL. 
596 |a 1 6 
655 7 |a Theses, IIUM local 
690 |a Dissertations, Academic  |x Department of Basic Medical Sciences  |z IIUM 
710 2 |a International Islamic University Malaysia.  |b Department of Basic Medical Sciences 
856 4 |u https://lib.iium.edu.my/mom/services/mom/document/getFile/ENs5ic5vdlD4uYopiuw53hhLsXMvaNaS20160322151404639  |z Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. 
900 |a hm-lfr 
999 |c 437777  |d 470154 
952 |0 0  |6 T RA 001242 P56 Z26E 2015  |7 0  |8 THESES  |9 760506  |a KIMC  |b KIMC  |c CLOSEACCES  |g 0.00  |o t RA 1242 P56 Z26E 2015  |p 11100341207  |r 2019-09-04  |t 1  |v 0.00  |y THESIS 
952 |0 0  |6 TS CDF RA 1242 P56 Z26E 2015  |7 0  |8 THESES  |9 853584  |a IIUM  |b IIUM  |c MULTIMEDIA  |g 0.00  |o ts cdf RA 1242 P56 Z26E 2015  |p 11100341208  |r 2017-10-26  |t 1  |v 0.00  |y THESISDIG