rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Microencapsulation and characterization of Gentamicin-PLGA microsphere intended for orthopaedic infection
  name:
    name part: Ahmad Fahmi bin Harun@Ismail
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Kulliyyah of Pharmacy
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2012
  language:
    language term: eng
  language:
    language term: -ma
  language:
    language term: y
  physical description:
    extent: [xix], 136 leaves : illustrations ; 30 cm.
  abstract: The study was done for the purpose of developing biodegradable gentamicin-loaded microspheres fabricated using poly(D.L-lactic-co-glycolic acid) (PLGA). The microspheres were fabricated by manipulating several variables i.e. molecular weight of PLGA, types of surfactant/emulsifier, different concentrations of polyvinyl alcohol (PVA) as well as the oil phase and different HLB values of surfactants in order to achieve the best formulation for W /0/W emulsion during the fabrication process. Antibiotic treatment of orthopaedic infection is complicated by systemic toxicity and the need of effective therapeutic concentration necessary to ensure optimum killing of bacteria. To overcome the problem of systemic toxicity and to achieve a high initial release followed by sustained release of antibiotics, a new method of delivering gentamicin is attempted by encapsulating gentamicin into PLGA using multiple emulsion, solvent-evaporation method. Gentamicin was first extracted from the microspheres and quantified using Ninhydrin assay before the concentration was measured using UV spectrophotometer. Gentamicin loading after encapsulation was preserved when CTAB (83.51 ± 1.42%) and low molecular weight (LMW) PLGA (82.38 ± 9.08%) were used as indicated by drug loading of more than 80% in the discdiffusion assay. LMW PLGA enabled high burst release (-90%) of gentamicin within the first 10 hours corresponding to zone inhibition of 13.78 ± 0.86 mm, only 30% smaller than the positive control (10 mg/ml gentamicin). The effects of Tg and molecular weight rather than surfactant types influence the initial burst release. The in vitro release profile suggests that by having a mixture of various PLGA microspheres in one dosage implant system, the high burst release can be sustained within therapeutic concentration for a prolonged period (> 1 months). This biodegradable delivery system does not entail another surgery to remove the implant hence reducing the high treatment cost usually associa…
  note: by Ahmad Fahmi bin Harun@Ismail
  note: Abstracts in English and Arabic.
  note: 'A dissertation in fulfilment of the requirement for the degree of Master in Pharmaceutical Science (Pharmaceutical Technology)' --On title page.
  note: Thesis (MSPHT) -- International Islamic University Malaysia, 2012.
  note: Includes bibliographical references (leaves 124-134).
  subject:
    geographic code: a-my---
  subject:
    topic: Musculoskeletal system
    topic: Infections Treatment
  subject:
    topic: Gentamicin
  subject:
    topic: Microencapsulation
  classification: RC925.5
  identifier: http://studentrepo.iium.edu.my/handle/123456789/5960
  record info:
    record content source: UIAM
    record creation date: 181023
    language of cataloging:
      language term: eng