Se2+ added carica papaya extract increases protection against oxidative damage and reduces inflamation during cutaneous wound repair /
Wounds due to injury or accident have become a major public health concern for the diabetic patient. Although, studies have led to new therapeutic interventions in wound healing, delayed wound healing still is a major burden. In more recent times, researchers have explored the use of plant extracts...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur:
Kulliyyah of Science, International Islamic University Malaysia,
2013
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Wounds due to injury or accident have become a major public health concern for the diabetic patient. Although, studies have led to new therapeutic interventions in wound healing, delayed wound healing still is a major burden. In more recent times, researchers have explored the use of plant extracts in combination with synthetic drugs or pure chemicals to expedite wound healing. Unripe Carica papaya Linn (papaya) is widely used in developing countries as an effective and readily available treatment for various wounds, particularly burns and chronic ulcers. Aseptic environment, reduced oxidative damage and increased cellular proliferation during tissue repair and regeneration are paramount to rapid and successful wound healing. Therefore, Se2+, an antioxidant co-factor and Zn2+, a component of signalling pathway for cellular proliferation were added to papaya extracts to analyze their potential in wound healing. This study was designed to investigate the combinatorial effect of Se2+ or Zn2+ and unripe papaya pulp extracts on the healing of full thickness excision wound in rodents. Extracts are prepared either in phosphate buffered saline (PBS) or water as solvent hence named as PE and WE respectively. Firstly, investigation was carried out on the mineral element composition, phytochemical classes, and SDSPAGE protein profile of the unripe papaya pulp extracts. Alkaloids were detected in both the PE and WE but tannin was detected in WE only. Ca (in the range of 106.89- 155.60 mg/100g extract) was present as the most abundant macro element as well as Na, Mg, and Fe in lesser quantities, while Se (in the range of 15.7-32.14 mg/100g extract) was found as the most abundant trace element with Mn, Zn and Ag detected in very small quantity. A number of protein bands of different intensities were observed on PE and WE lane of SDS-PAGE. The high Mr bands (> 35 KDa) were resolved with low intensity in both PE and WE. Protein band with Mr ~ 27 KDa was present in PE but absent in WE. Moderate Mr bands ~ 24 and 19 KDa were resolved in PE and WE however with higher intensity of band ~ 19 KDa and protein fragments observed on PE lane. Total yield of 6.7 % was recovered from PE which contains 9% protein while WE yields 9.2 % total which contains 0.58 % protein. In an attempt to analyse an effective concentration of either Se2+ or Zn2+ required for improved wound healing, different concentrations of Se2+ and Zn2+ were combined with PE and WE. Full thickness excision wound induced on mice were treated with PE or WE in combination with or without the selected concentrations (Zn2+ 100 & 200 mM, Se2+ 3.17 × 10-1 & 6.34 × 10-1 mM) of the elements. Positive control group (PC) was treated with solcoseryl and negative control (NC) was treated with sterile deionised water. Wound area was monitored by a digital camera and wound size measured using Adobe photoshop CS5 Extended (Version 12.0.4 x 32: Adobe, Inc.). Se2+ added PE at 0.5 or 1.0 g took ~ 9 days while control groups took 14 days and other treatment groups took at least 10 days for the complete wound closure. The wound healing efficiency calculated from the time taken for complete wound closure was significantly (p < 0.05) higher for Se2+ (3.17 × 10-1 or 6.34 × 10-1 mM) added PE compared to other groups. Biochemical assays, histochemical and immunohistochemical methods were used to examine the mechanisms of wound repair and reduced inflammation in rats induced with excision wound after treatment with Se2+ (3.17 × 10-1 mM) or Zn2+ (100 mM) added papaya extracts. Again Se2+ added PE at 3.17 × 10-1 mM took 8.90 days on average for complete wound closure |
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| Physical Description: | xxi, 240 leaves : ill. ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 181-219). |