Effect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applications /
An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa oil (NSO) in a form of gentamicin-N. sativa fusion emulsion (GNFE). This work aims to formulate a stable emulsion and to study the effects of GNFE on UMR-106 osteoblast-li...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
Kuantan, Pahang :
Kulliyyah of Allied Health Sciences, International Islamic University Malaysia,
2016
|
| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| LEADER | 030580000a22002770004500 | ||
|---|---|---|---|
| 008 | 160205t2016 my a g m 000 0 eng d | ||
| 040 | |a UIAM |b eng | ||
| 041 | |a eng | ||
| 043 | |a a-my--- | ||
| 050 | 0 | 0 | |a RM666.G44 |
| 100 | 0 | |a Fathin Athirah binti Yusof | |
| 245 | 1 | |a Effect of gentamicin-nigella sativa fusion emulsions on osteoblast cell line for use in osteo healing-applications / |c byFathin Athirah binti Yusof | |
| 260 | |a Kuantan, Pahang : |b Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, |c 2016 | ||
| 300 | |a xxvi, 165 leaves : |b ill. ; |c 30cm. | ||
| 502 | |a Thesis (MHSC)--International Islamic University Malaysia, 2016 | ||
| 504 | |a Includes bibliographical references (leaves 148-162) | ||
| 520 | |a An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa oil (NSO) in a form of gentamicin-N. sativa fusion emulsion (GNFE). This work aims to formulate a stable emulsion and to study the effects of GNFE on UMR-106 osteoblast-like rat osteosarcoma cell line in vitro and its related mechanisms of bone healing and regeneration. Emulsion A, B, C and D had been formulated, with final concentration of gentamicin was made constant at 0.1%, whereas NSO concentration was varied at 32.5%, 35.0%, 40.2% and 46.4% in all formulations respectively. Stability studies of emulsion A, B, C and D were performed at different storage conditions (8°C, 25°C and 50°C), followed by in vitro study of MTT assay, Alizarin Red S (ARS) staining, von Kossa staining and quantification, alkaline phosphatase (ALP) quantification and quantitation of collagen type-1 and osteocalcin (qPCR). Results showed that all emulsions were stable at storage temperature of 8°C. In vitro results showed that emulsion D produced the highest cell viability (97.1%) at 72 hours of post-incubation. The highest mineral deposits (2.64 ± 0.05) and ALP activity (2.19 ± 0.3 nmol) was produced by emulsion D at day 21. Lastly, the highest expression of collagen type-1 (29.4 ± 1.01 folds) and osteocalcin (1.8 ± 0.51 folds) were expressed by the cells treated with emulsion C. Thus, stable GNFE may have the ability to promote bone formation. | ||
| 596 | |a 1 6 | ||
| 655 | 7 | |a Theses, IIUM local | |
| 690 | |a Dissertations, Academic |x Department of Biomedical Science |z IIUM | ||
| 710 | 2 | |a International Islamic University Malaysia. |b Department of Biomedical Sciences | |
| 856 | 4 | |u https://lib.iium.edu.my/mom/services/mom/document/getFile/VGiiRBiySduqV9zweh343uJLLmhVgj2n20160329145550469 |z Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. | |
| 900 | |a snas-sbh-ls | ||
| 999 | |c 438564 |d 468776 | ||
| 952 | |0 0 |6 T RM 000666 G44 F252E 2016 |7 0 |8 THESES |9 760901 |a KIMC |b KIMC |c CLOSEACCES |g 0.00 |o t RM 666 G44 F252E 2016 |p 11100344023 |r 2019-09-04 |t 1 |v 0.00 |y THESIS | ||
| 952 | |0 0 |6 TS CDF RM 666 G44 F252E 2016 |7 0 |8 THESES |9 854211 |a IIUM |b IIUM |c MULTIMEDIA |g 0.00 |o ts cdf RM 666 G44 F252E 2016 |p 11100344024 |r 2017-10-26 |t 1 |v 0.00 |y THESISDIG | ||