Evaluation of mismatch repair and microsatellite instability in colorectal cancer patients in Kuantan, Pahang /

Colorectal cancer (CRC) is the second most common tumour in Malaysia. Universal screening for the identification of microsatellite instability/mismatch repair (MSI/MMR) status in CRC patients is recommended by several guidelines. The detection of MSI/MMR status in CRC patients is not only essential...

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Bibliographic Details
Main Author: Muhammad Ishaque Faizee (Author)
Format: Thesis
Language:English
Published: Kuantan, Pahang : Kulliyyah of Medicine, International Islamic University Malaysia, 2021
Subjects:
Online Access:http://studentrepo.iium.edu.my/handle/123456789/10782
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040 |a UIAM  |b eng  |e rda 
041 |a eng 
043 |a a-my--- 
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100 0 |a Muhammad Ishaque Faizee,  |e author  |9 3861 
245 1 0 |a Evaluation of mismatch repair and microsatellite instability in colorectal cancer patients in Kuantan, Pahang /  |c by Muhammad Ishaque Faizee 
264 1 |a Kuantan, Pahang :  |b Kulliyyah of Medicine, International Islamic University Malaysia,  |c 2021 
300 |a xviii, 150 leaves :  |b colour illustrations ;  |c 30cm. 
336 |2 rdacontent  |a text 
337 |2 rdamedia  |a unmediated 
337 |2 rdmedia  |a computer 
338 |2 rdacarrier  |a volume 
338 |2 rdacarrier  |a online resource 
347 |2 rdaft  |a text file  |b PDF 
500 |a Abstracts in English and Arabic. 
500 |a "A thesis submitted in fulfilment of the requirement for the degree of Master of Medical Sciences." --On title page. 
502 |a Thesis (MMDSC)--International Islamic University Malaysia, 2021. 
504 |a Includes bibliographical references (leaves 116-145). 
520 |a Colorectal cancer (CRC) is the second most common tumour in Malaysia. Universal screening for the identification of microsatellite instability/mismatch repair (MSI/MMR) status in CRC patients is recommended by several guidelines. The detection of MSI/MMR status in CRC patients is not only essential to identify Lynch Syndrome (LS), but it also has predictive and prognostic values. The study aimed to investigate the MMR and MSI status among CRC patients in Kuantan, Pahang as well as to assess the consistency between immunohistochemistry (IHC) and MSI analysis. Formalin-fixed paraffin-embedded (FFPE) tissue blocks of 123 CRC patients were retrieved for the years 2017-2018. For IHC and MSI analysis, EnVisionTM FLEX, Mini Kit, High PH, and MSI Analysis System 1.2 (Promega) were utilized, respectively. MSI analysis was performed on selected deficient mismatch repair (dMMR) and proficient mismatch repair (pMMR) cases. IHC detected 11.4% (14 out of 123) patients as dMMR and 88.6% (109 out of 123) as pMMR. MSI analysis identified 26% (13 out of 50) patients as MSI-H, 6% (3 out of 50) patients as MSI-L, and 64% (32 out of 50) patients as MSS. Both the IHC and MSI analysis showed perfect agreement (0.896, Kappa value) for the recognition of dMMR or MSI-H CRC patients while demonstrating only 4% (2 out of 50) discordant results. Almost all dMMR patients detected by IHC were recognized by MSI analysis as MSI-H except one. The significant prevalence of dMMR in current cohort support the recommendation that the assessment of MSI/MMR status should be addressed in CRC patients. The selection of the choice method may be based on the availability of the expertise and equipment. Since IHC is an affordable, a reproducible and readily available in most histopathological laboratories, it can be used as a primary screening test to detect MSI/MMR status in CRC patients. 
650 0 |a Colon (Anatomy)  |x Cancer  |z Malaysia  |9 3862 
650 0 |a Colon (Anatomy)  |x Diseases  |x Diagnosis  |z Malaysia  |9 3863 
655 7 |a Theses, IIUM local   |9 3864 
690 |a Dissertations, Academic  |x Department of Pathology and Laboratory Medicine  |z IIUM  |9 3865 
700 0 |a Asmah Hanim Hamdan,  |e degree supervisor  |9 3866 
700 0 |a Norlelawati A. Talib,  |e degree supervisor  |9 3867 
710 2 |a International Islamic University Malaysia.  |b Department of Pathology and Laboratory Medicine  |9 3868 
856 4 |u http://studentrepo.iium.edu.my/handle/123456789/10782 
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