rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Electrosprayed crispr plasmid DNA loaded alginate nanoparticles
    sub title: preparation, characterisation, and gene editing
  name:
    name part: Alallam, Batoul
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Department of Pharmaceutical Technology
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2021
  language:
    language term: eng
  physical description:
    extent: xxi, 205 leaves : colour illustrations ; 30cm.
  abstract: Background: CRISPR/ Cas9 is one of the most powerful among the approaches being developed to rescue fundamental causes of gene-based inheritable diseases. Several strategies for delivering such genome editing materials have been developed, but the safety, efficacy over time, cost of production, and gene size limitations are still under debate and must be addressed to further improve applications. Sodium alginate is frequently used as the model encapsulation matrix for bioactive ingredients in the field of drug and gene delivery due to its safety. Objective: To encapsulate CRISPR/Cas9 plasmid DNA in alginate nanocarrier to perform genome editing. Methodology: alginate nanoparticles loaded with two CRISPR pDNA were fabricated using electrospray method. Both formulation and process were optimised. Chitosan-, Arabic gum- and PEG-coated CRISPR-loaded alginate nanoparticles were fabricated and characterised. CRISPR-loaded alginate nanoparticles physicochemical properties were compared to the surface-modified nanoparticle properties. The influence of surface modification of nanoparticles on their interaction with cell was studied in regard to cellular uptake, cytotoxicity, transfection efficiency, and genome editing. Results: Using electrospray, a nanoparticle carrier was developed to deliver CRISPR pDNA into HepG2 cells. The nanoparticles size was approximately 230 nm, with an encapsulation efficiency of 99%. Release study revealed that over one-third of the pDNA was released within the first 24 h. In vitro experiments conducted with HepG2 cells demonstrated that after 48 h of treatment with the CRISPR-loaded alginate nanoparticles, the particles were not toxic. CRISPR-loaded alginate nanoparticles mediated 1.5-folds more efficient transfection than a commercially available cationic liposome-based transfection reagent. However, their indel efficiency was 3.4-folds lower than the transfection reagent. The surface coating highly affected the nanoparticles physicochemical pr…
  note: by Batoul Alallam
  note: Thesis (MSPHT)--International Islamic University Malaysia, 2021.
  note: Includes bibliographical references (leaves 168-192).
  subject:
    geographic code: a-my---
  identifier: http://studentrepo.iium.edu.my/handle/123456789/10638
  record info:
    record content source: UIAM
    record creation date: 210921
    language of cataloging:
      language term: eng