Anticancer study of Neolamarckia Cadamba (Kalempayan) leaves extract on breast cancer carcinoma cells (MCF-7) /
Breast cancer is a leading cause of cancer-related death in women. Despite the advancement of breast cancer treatments, patients are suffered with side effects and other consequences such as cancer recurrence and resistance to treatments. An effective strategies are needed of which resources from th...
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| Format: | Thesis |
| Language: | English |
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| Online Access: | http://studentrepo.iium.edu.my/handle/123456789/9585 |
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| Summary: | Breast cancer is a leading cause of cancer-related death in women. Despite the advancement of breast cancer treatments, patients are suffered with side effects and other consequences such as cancer recurrence and resistance to treatments. An effective strategies are needed of which resources from the natural plants have been used for alternative approach as anti-cancer agents against breast cancer. The present study is an attempt to evaluate anti-cancer activities of Neolamarckia cadamba leaf (NCL) extract on the breast cancer carcinoma cells (MCF-7). MCF-7 cells were treated with various concentration of ethanolic NCL extracts. The concentration of NCL extract that caused 50% of cell growth inhibition [IC50] in MCF-7 cells was determined after 72 hours of treatments, and its anti-proliferative effect was assessed upon treatment for 24, 48, 72 and 96 hours using TBEA method. NCL extract demonstrated inhibitory activity in cell growth at IC50 value of 0.206 mg/ml, in dose-dependent and time-independent manner. The effect of NCL extract on apoptosis induction and cell cycle arrest were analysed using flow cytometer. The flow cytometric analysis indicated that NCL extract inhibits the growth of MCF-7 cells by inducing apoptosis and cell cycle arrest in G0/G1 phase. Meanwhile, gene expression analysis of qPCR assay revealed NCL extract induced apoptosis through the down-regulated of Bcl-2, whereas Bax cytochrome c, caspase-9 and caspase-7 were up-regulated in MCF-7 cells. Cell cycle arrest was associated with down-regulated of CDK2 with subsequent up-regulated of p21 and cyclin E. Overall, our data demonstrated that the NCL extract exerts anti-cancer effect on MCF-7 human breast cancer cells through induction of apoptosis and cell cycle arrest. The present study suggests that NCL extract may be an important alternative of anti-cancer agent candidate in breast cancer treatment. |
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| Item Description: | Abstracts in English and Arabic. "A thesis submitted in fulfilment of the requirement for the degree of Master of Health Sciences." --On title page. |
| Physical Description: | xvii, 141 leaves : illustrations ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 115-129). |