rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Evaluation of antidiabetic and antioxidant properties and metabolite profiling of Momordica charantia fruit using metabolomics approach
  name:
    name part: Vikneswari Perumal
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Kulliyyah of Pharmacy
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2018
  language:
    language term: eng
  physical description:
    extent: xxi, 298 leaves : colour illustrations ; 30cm.
  abstract: Momordica charantia Linn (Cucurbitaceae) has been widely commercialized based on traditional usage as an antidiabetic product. However, the scientific evidence of its antidiabetic activity is not sufficient. Hence, the major aims of this research were to evaluate the antidiabetic activity of M. charantia fruit through proton-nuclear magnetic resonance (1H-NMR) spectroscopy based metabolomics, to investigate its mechanism of action, to profile the identified antioxidants as antidiabetic agents through liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) based metabolomics, and to develop a validated regression model using fourier transform infra-red spectroscopy (FT-IR) based fingerprinting. Initially, the fruit was extracted by the solvent with different ethanol in water concentration (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were subjected to different in vitro assays. The 80% aqueous ethanolic extract possessing the highest in vitro bio-activity was chosen for further in vivo antidiabetic evaluation utilizing 1H NMR based metabolomics approach. In vitro dipeptidyl peptidase-4 (DPP-IV) inhibitory and 3T3-L1-cell glucose uptake activities were also tested for the same extract to explain its mode of action in relation to the metabolomics results. LC-MS and GC-MS based metabolomics approaches were applied to profile the bioactive compounds present in the extract. Finally, a validated calibration model was developed using FT-IR based fingerprinting. The 80% ethanolic extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, but failed to exhibit inhibitory activity against α-glucosidase and xanthine oxidase enzymes. Thereby, the antidiabetic activity of this extract was further evaluated using streptozotocin obese-diabetic induced rats (STZ ob-db). The results showed that the administration of the 80% ethanolic extract at 300 mg/kg bw for 4 …
  note: by Vikneswari a/p Perumal
  note: Thesis (Ph.D)--International Islamic University Malaysia, 2018.
  note: Includes bibliographical references (leaves 162-190).
  subject:
    geographic code: a-my---
  classification: QK495.C96
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/5XFJAHgzaql3wiHlvo7yH8oxJ56hgjKt20180726121736298
  record info:
    record content source: UIAM
    record creation date: 180530
    language of cataloging:
      language term: eng