rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Development of analytical methods for the determination of amlodipine, gliclazide and perindopril in dosage forms and plasma
  name:
    name part: Alaama, Mohamed
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Kulliyyah of Pharmacy
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2017
  language:
    language term: eng
  physical description:
    extent: xxx, 269 leaves : illustrations ; 30cm.
  abstract: Generic formulation is getting popular world-wide. Demand for reliable analytical and bio analytical technique is getting more attention from the researchers around the globe. A total analytical and bio analytical solution for generic formulations are reported in this study. Analytical and bioanalytical methods were developed and validated for the determination of antihypertensive drugs (Amlodipine (AMLO) and perindopril (PER)) and antidiabetics drug (gliclazide (GLZ)) in pharmaceutical formulations and human plasma using HPLC and LC-MSMS. Firstly, new sensitive and economic analytical method was developed and validated for the analysis of AMLO in tablet dosage form using High Performance Liquid Chromatography (HPLC) with UV detector. The degradation profile of AMLO was identified and the degradation diagram was created. In second part, an HPLC with Diode Array Detector (DAD) was developed and validated for the determination of AMLO in human plasma with cetirizine (CTZ) as internal standard. Sample preparation was optimized using DOE (Design of Experiment). Three different parameters namely solvent type, solvent volume and pH were monitored at different levels for the optimization of sample preparation that would produce best recovery of the analytes. Chromatographic separation of the analytes was obtained using Phenomenix C18 (150 × 4.5 mm, 5 µm) column. The third method was the analysis of GLZ in tablet dosage form using HPLC instrument with modern rapid resolution column. Samples were injected to C18 Waters Xselect HSS C18 (2.1 × 100 mm, 2.5 µm) column. The method showed high recovery, precision and repeatability. The degradation products were identified and some products were reported for the first time. In fourth part, we developed and validated new LC-MSMS method for the determination of GLZ in rats' plasma using CTZ as internal standard. The method was developed using Aquity Waters H Class UPLC connected to Xevo TQD MS detector. The total run time was 5 min w…
  note: by Mohamed Alaama
  note: Thesis (Ph.D)--International Islamic University Malaysia, 2017.
  note: Includes bibliographical references (leaves 253-266).
  subject:
    geographic code: a-my---
  classification: RS55.2
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/udkqlOvuPqZCgUGaWyRuJg3xfEtC0aWb20180118115353770
  record info:
    record content source: UIAM
    record creation date: 171011
    language of cataloging:
      language term: eng