Star-shaped polycaprolactone-poly(ethylene glycol) block copolymer for ciprofloxacin delivery /
Most drugs used in treating disease are hydrophobic. This property gives drawbacks since human body generally easily absorbs hydrophilic compounds. An amphiphilic drug delivery system which consists of hydrophobic core and hydrophilic outer shell may overcome this problem. In our work, two types of...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
Kuantan, Pahang :
Kulliyyah of Sciences, International Islamic University Malaysia,
2018
|
| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Most drugs used in treating disease are hydrophobic. This property gives drawbacks since human body generally easily absorbs hydrophilic compounds. An amphiphilic drug delivery system which consists of hydrophobic core and hydrophilic outer shell may overcome this problem. In our work, two types of star-shaped amphiphilic co-polymers were synthesized and characterized for drug delivery system for wound healing application. Four and six arms initiators were used in preparing polycaprolactone, (PCL) hydrophobic core with molecular weight of approximately 40 kDa via ring opening polymerization. Stannous octoate was used as the catalyst in ring opening polymerization of PCL and the reaction was conducted at 100 °C to reduce the formation of side products. NMR and FTIR analyses showed that ring opening of ԑ-caprolactone had occurred producing four and six arms star homopolymer PCL. Further reaction of these two homopolymers with succinilated methoxy poly(ethylene glycol), (mPEG) produced amphiphilic star-shaped polymer consisted of polycaprolactone-poly(ethylene glycol). NMR and FTIR analyses showed that the mPEG was successfully attached to the PCL arms in both star-shaped polymers. Thermal analysis of the product using DSC gave the crystalline melting point, Tm ranged from 56-60 °C. However, no significant difference in Tm for both four and six arms co-polymers was observed, indicating that the architecture does not significantly affect the thermal behavior. Both star-shaped copolymers were incorporated into hydrogel formulation to investigate their ability as drug delivery system. The homopolymer star PCL could not form homogenous hydrogel and tend to agglomerate in the system. However, the presence of PEG in the outer shell helped to produce homogenous hydrogel. The presence of PCL in both formulations showed high drug entrapment efficiency (>95%) via hydrophobic-hydrophobic interaction with the drug. Drug release study of hydrogel containing six arms co-polymers showed slightly higher amount of drug release compared to hydrogel with four arms co-polymers. This is due to the presence of PEG that increased the water uptake of the molecule leading to faster drug release rate. However, both hydrogels showed sustained drug release pattern, signifying the drug release was due to diffusion of PCL. Kinetic drug release study of the hydrogels indicate that both formulations are best fitted in Korsmeyer-Peppas released model. Both formulations shows antibacterial activity towards E. coli, P. aeruginosa, E. faecalis and S. pyogenes associated to the antibacterial activity of ciprofloxacin. |
|---|---|
| Physical Description: | xvii, 136 leaves : illustrations ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 94-112). |