Comparison of monoculture and co-culture of microalgae for enhanced lutein production /
Single species cultivation or monoculture is a classic method used in the industries to improve intracellular and/or extracellular production from the living organisms. However, the problem of monocultures is that they are unstable and prone to perturbation by bacteria and fungi. These contaminants...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Engineering, International Islamic University Malaysia,
2017
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| Subjects: | |
| Online Access: | http://studentrepo.iium.edu.my/handle/123456789/4414 |
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| Summary: | Single species cultivation or monoculture is a classic method used in the industries to improve intracellular and/or extracellular production from the living organisms. However, the problem of monocultures is that they are unstable and prone to perturbation by bacteria and fungi. These contaminants can easily grow and invaded the population while hampering the growth of the target species. Co-cultivation of two or more species has shown to improve the stability and immunity of the culture. Their symbiotic interaction by competition or cooperation has also proven to increase the biomass and productivity. This method of cultivation has brought plentiful success to the industrial applications such as wastewater treatments and biofuel. By co-culturing the microalgae, the present study intends to improve the productivity of lutein, one of the bioactive compounds of microalgae that is popularly known in treating eye disease related to aging. To conduct this experiment, several indigenous isolates of green microalgae from Malaysia freshwater lakes together with other microalgae strains from two different culture collection centres were first screened to assess their potential as the lutein producer. Three highest lutein producer strains were then selected for co-cultivation study in which several ratios were screened to obtain culture with an equally growing number of cells. Only one combination at one ratio producing an equal number of cells was analyzed for lutein content. The lutein content of monoculture and co-culture were then compared. Preliminary investigations showed that lutein was detected in all six strains of algae. Chlorella sp. B, Scenedesmus dimorphus and Ankistrodesmus sp. TH were the top three highest lutein producers and these strains were further investigated for the co-cultivation study. Screening of different combinations of the three strains for co-cultivation study demonstrated that co-culture of S. dimorphus and Ankistrodesmus sp. TH at a ratio of 1 to 2 prevented the domination of one microalga over the other with higher lutein content (38.66 mg per g of dry biomass) than those obtained in the other combinations. Nevertheless, lutein content in the aforementioned co-culture is not as high as the amount achieved in the monoculture of S. dimorphus (60.11 mg per g of dry biomass). In conclusion, for lutein production, monoculture cultivation is better than co-culture cultivation unlike for the production of biofuel and waste treatment. |
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| Physical Description: | xv, 81 leaves : illustrations ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 72-79). |