rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Evaluation of three-dimensional poly (lactic-co-glycolic acid), atelocollagen and fibrin scaffolds for intervertebral disc tissue engineering
  name:
    name part: Mohd Yusof bin Mohamad
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Kulliyyah of Allied Health Sciences
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2017
  language:
    language term: eng
  physical description:
    extent: xxiv, 204 leaves : illustrations ; 30cm.
  abstract: Tissue engineering and regenerative medicine (TERM) offers an alternative approach for the treatment of intervertebral disc (IVD) degeneration. One of the important TERM principles is the application of biomaterial scaffolds. Poly(lactic-co-glycolic acid) (PLGA) is a US Food and Drug Administration (FDA) approved synthetic copolymer for clinical use. However, for TERM application, PLGA needs to be improved. Natural polymers, for example atelocollagen and fibrin can be used to enhance the PLGA properties. The combination of PLGA with atelocollagen and fibrin for IVD regeneration has not been explored yet. The aim of the present study is to evaluate firstly the monolayer culture of IVD cells, secondly the PLGA-based scaffolds namely PLGA alone, PLGA with atelocollagen (PA), PLGA with fibrin (PF) and PLGA with the combination of both atelocollagen and fibrin (PAF), thirdly the in vitro 3D constructs engineered from PLGA-based scaffold seeded with the annulus fibrosus (AF) cells and fourthly the in vivo constructs implanted subcutaneously on the athymic nude mice model. Important Islamic principles in relation with the IVD regeneration were also discussed as the fifth objective. Growth kinetics analysis and cellular proliferation assay were performed on the monolayer cells culture and in vitro constructs at day 4, 7, 14 and 21, respectively. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to evaluate cell morphology both in monolayer and the in vitro 3D culture. The fabricated PLGA- based scaffolds were characterized in terms of chemical bonding, porosity, water uptake, thermal, mechanical, degradation, pH level and compression modulus. Other evaluation parameters included histology, immunohistochemistry, gene expression, sulphated glycosaminoglycan (sGAG) and DNA quantification analysis. All constructs were evaluated at 1, 2 and 3 weeks in vitro and, at 4 weeks in vivo. The presence of autophagasome in AF cells was detected in al…
  note: by Mohd Yusof bin Mohamad
  note: Thesis (Ph.D)--International Islamic University Malaysia, 2017.
  note: Includes bibliographical references (leaves 163-182).
  subject:
    geographic code: a-my---
  classification: R857.T55
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/iEq1ylaca7JGtWVjJbj4x2XTZpWMBEuu20180419105315753
  record info:
    record content source: UIAM
    record creation date: 180416
    language of cataloging:
      language term: eng