Enzymatic hydrolysis of sago wastewater sediment for glucose production /
Sago wastewater contains a high amount of organic compounds that can pollute the environment. Fortunately, the wastewater can be utilized as a substrate for fermentation process. However, polysaccharides in the effluent need to be broken down by the mean of hydrolysis. Many studies have been conduct...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Kuala Lumpur :
Kulliyyah of Engineering, International Islamic University Malaysia,
2018
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| Subjects: | |
| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Sago wastewater contains a high amount of organic compounds that can pollute the environment. Fortunately, the wastewater can be utilized as a substrate for fermentation process. However, polysaccharides in the effluent need to be broken down by the mean of hydrolysis. Many studies have been conducted to hydrolyze starch enzymatically but few have focused on the process parameters. Thus, this study aimed to identify the significant factors that affecting enzymatic hydrolysis of sago wastewater sediment. Eight factors namely α-amylase concentration (0.05-0.20 % v/v), glucoamylase concentration (0.05-0.20 % v/v), substrate concentration (0.5-1.0 % v/v), initial pH (4-8), liquefaction temperature (70-90 °C), liquefaction reaction time (60-120 min), saccharification temperature (50-70 °C) and saccharification reaction time (24-48 h) were chosen for screening using Plackett-Burman design. The ANOVA result showed that α-amylase concentration, glucoamylase concentration and saccharification temperature were the three most significant factors affecting glucose yield and were chosen for further optimization. The possible optimum level of factors for glucose production was determined by using One-Factor-at-a-Time (OFAT) method. This step was conducted to determine the range of selected factors for subsequent Face-Centered Central Composite Design (FCCCD). Later it was found that the optimum level for α-amylase concentration, glucoamylase concentration and saccharification temperature were 0.02%, 0.3% and 50°C, respectively. Next, these selected factors were included in FCCCD to find the optimum level of factors that give the maximum glucose yield. The maximum glucose yield of 69.48% was obtained when glucoamylase concentration, saccharification temperature and α-amylase concentration were set to 0.3%, 40°C and 0.01%, respectively. Result from the statistical analysis revealed that only glucoamylase concentration and saccharification temperature were significantly affecting the enzymatic hydrolysis. Finally, validation test was performed where the percentage error between experimental (69.48%) and predicted (64.83%) glucose yield was only 7%, hence confirmed the validity of the statistical model. |
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| Physical Description: | xii, 76 leaves : colour illustrations ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 63-68). |