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Core-shell Ag@Pt metalic nanoparticles as a targeted drug carrier for cancer treatment
International Islamic University Malaysia
Cancer is a complicated disease for which finding cure presents challenges. In recent decades new ways to treat cancer are being sought; one being nanomedicine which manipulates nanoparticles to target a cancer and release drugs directly to the cancer cells. A number of cancer treatments based on nanomedicine are under way and mostly are in preclinical trials owing to challenges in administration, safety, and effectiveness. One new method for drug delivery is the use of core-shell (metal-metal) nanoparticles, which has the potential to deliver drugs via light activation. In this study, a comparative study of the effects were carried out on Vero cell, a normal cell line. Results showed that, the two type of extracts inhibits growth of MCF-7 cell at varying degrees, but on the other hands, that have much less effect on Vero cell. Extracts, identified using Raman Spectroscopy shows peaks at 1349 cm-1, 944 cm-1 and 841 cm-1 indicating the presence of Tyr, Try and Gly. In addition, UV-vis analysis showed peaks in the range of 260 to 280 nm, inferring the presence of Tyr and Try, whereas the highest peak, 3.608 at 230 nm indicates the presence of peptide bond. In order to synthesis core-shell Ag@Pt NPs as photovoltaic drug carrier, the process parameters of AgNPs, Ag@Pt NPs and drug loaded Ag@Pt NPs synthesis has been statistically optimized by first executing One-Factor-At-a-Time (OFAT) strategy on three important parameters, this was followed by executing CCD design of experiments and ANN, to find the optimized parameters and the interactions among them, for maximal NPs production. The NPs sample was then characterized by FESAM, TEM, HRTEM, FTIR and UV-vis analysis. The performance test of peptide coated Ag@Pt NPs characterization was done by checking the drug releasing ability using with and without UV together with checking the biological interaction with MCF 7, breast cancer cell. Results showed that, the optimal and maximal of AgNPs production condition for both ANN and RSM was 63 Ag NPs synthesis are 1% TSC concentration, pH value 7, 3.5 min of reaction time and 1mM AgNO3 concentration and, the both methodologies were used to determine statistically the optimized process conditions based on AgNPs yield, analyzed via UV-vis analysis in the range of 350 to 420 nm wavelength. Further, 8% chloroplatinic acid and Ag NPs absorbance at 1.6 @ 420 nm wavelength were the optimized condition obtained for both models, which is calculated using the area under the curve from UV-vis analysis in the wave length range of 300 nm to 350 nm. In addition with, the maximum experimental drug loaded Ag@Pt NPs yield was 3.8 AU and was found at the same conditions for both ANN and RSM developed model. In addition, both methodologies were compared for their modelling, sensitivity analysis and optimization ability, as well as construct there response/output surface plot in order to determine the influences and interactions of input variables. The UV light used for drug release was rather weak in intensity, therefore, it is not able to kill or cause any harm to the cancerous cells but sufficient to enhance the photodynamic effects of the drug carrier Ag@Pt NPs to release drug and kill the cancer cells.
Thesis (MSMCT)--International Islamic University Malaysia, 2017.
Includes bibliographical references (leaves 209-225).