rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Bioassay guided isolation and characterization of compounds with antioxidant activities from the leaves of Entada spiralis Ridl. (Sintok)
  name:
    name part: Sharifah Nurul Akilah binti Syed Mohamad
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Department of Pharmaceutical Chemistry
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2017
  language:
    language term: eng
  physical description:
    extent: xxii, 211 leaves : colour illustrations ; 30cm.
  abstract: Entada spiralis Ridl. known as Sintok or Akar Beluru is the woody climber plant belong to Leguminoceae family. E. spiralis is an excellent foaming agent due to the presence of saponin. Thus, E. spiralis stem bark is established used as natural body soap, shampoo and washing agent. The possession of antioxidant activity has been revealed by E. spiralis ability to treat syphilis, insect bites and superficial skin diseases. This research aims to screen the major compounds by phytochemical screening test, verify the antioxidant compounds from crude extracts and fractions via in-vitro antioxidant activity assays, isolate the antioxidant compounds through chromatographic methods and characterize the structure of antioxidant compounds by using various spectroscopic techniques. The crude extract of E. spiralis leaves was prepared by macerating the leaves with petroleum ether, chloroform, and methanol solvent sequentially. Determination and evaluation of antioxidant activity was done through in-vitro study by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation. Folin-Ciocalteu's reagent method was used to measure total phenolic content and flavonoid content was evaluated by calorimetric method. The crude extract of E. spiralis leaves was fractionate by vacuum liquid chromatography (VLC) and liquid-liquid extraction. Isolation of antioxidant compounds was further with column chromatography technique and characterized by spectroscopic analysis. Methanol, chloroform, and petroleum ether crude extracts were analyzed with 5.53 %, 2.05 % and 2.55 % yield. Saponin and flavonoid were the major components. Methanol extract exhibited the highest antioxidant activity with IC50 (DPPH); 40.23 ± 2.66 ug/mL and IC50 (ABTS); 5.09 ± 0.53 ug/mL. The highest possession of phenolic content and flavonoid content; 124.67 ± 6.63 mg GAE/g and 51.67 ± 2.17 mg QE/g were also displayed by the methanol extract. Isoscutellare…
  note: by Sharifah Nurul Akilah binti Syed Mohamad
  note: Thesis (MSPHC)--International Islamic University Malaysia, 2017.
  note: Includes bibliographical references (leaves 155-165).
  subject:
    geographic code: a-my---
  classification: QK495.M545
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/vbZhzXgVaC17OFZWf3deTGjGPY5q0jge20180426143706672
  record info:
    record content source: UIAM
    record creation date: 180418
    language of cataloging:
      language term: eng