rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Enrichment of novel antimicrobial compound from stereospermum fimbriatum using supercritical carbon dioxide extraction
  name:
    name part: Anis Fadhlina Izyani binti Awang
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: Sarker, Md. Zaidul Islam
    role:
      role term: degree supervisor
  name:
    name part: Ahmed, Qamar Uddin
    role:
      role term: degree supervisor
  name:
    name part: Juliana Md. Jaffri
    role:
      role term: degree supervisor
  name:
    name part: Muhammad Nor Omar
    role:
      role term: degree supervisor
  name:
    name part: International Islamic University Malaysia
    name part: Kulliyyah of Pharmacy
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2019
  language:
    language term: eng
  physical description:
    extent: xx, 171 leaves : colour illustrations ; 30cm.
  abstract: Stereospermum fimbriatum or “Chicha” (S. fimbriatum) is an undiscovered medicinal plant that is traditionally used for itchy skin, earache, stomachache and postpartum treatment. The present study was designed to evaluate antimicrobial properties of flowers, leaves, stem bark and twig of S. fimbriatum against eleven skin-associated pathogens as well as to isolate the bioactive compound from the active extract of S. fimbriatum and investigate its structure activity relationship by molecular docking. Supercritical carbon dioxide (sc-CO2) extraction was also carried out in order to obtain an enriched extract containing the isolated compound. The phytochemicals screening was done to determine the presence of alkaloids, terpenoids, flavonoids, steroids, saponins and tannins. Successive soxhlet extraction was conducted using n-hexane, dichloromethane (DCM) and methanol. Disc diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were done to examine the antimicrobial activity of the crude extracts. Bioassay-guided isolation of active compound was conducted by column and thin layer chromatographies. Molecular docking studies were done on five main targets for anti-MRSA activity, namely, penicillin binding proteins 2a, β-lactamase, DNA topoisomerase IV, dehydrosqualene synthase and sortase A. The binding sites and docking scores between a known inhibitor (positive controls) and an isolated compound were compared. Phytochemical screening revealed the presence of terpenoid, steroid, flavonoid, saponin and tannin in different parts of S. fimbriatum. The DCM extract of stem bark (DS) was found to be the most potent extract followed by n-hexane extract of the stem bark (NS) against Staphylococcus epidermidis, methicillin-resistant S. aureus (MRSA) and S. aureus with inhibition zones ranged from 13 to 16 mm. MIC values for NS and DS ranged between 400-800 µg/mL against S. epidermidis, MRSA and S. aureus. The bioassay-guided isolation …
  note: by Anis Fadhlina Izyani binti Awang
  note: Abstracts in English and Arabic.
  note: "A thesis submitted in fulfilment of the requirement for the degree of Doctor of Philosophy in Pharmaceutical Sciences (Pharmaceutical Technology)." --On title page.
  note: Thesis (Ph.D)--International Islamic University Malaysia, 2019.
  note: Includes bibliographical references (leaves 144-159).
  subject:
    topic: Materia medica, Vegetable
  subject:
    topic: Medicinal plants
    topic: Therapeutic use
  subject:
    topic: Skin
    topic: Infections Treatment
  subject:
    topic: Stereospermum fimbriatum
    topic: Therapeutic use
  classification: RS164.S74
  identifier: http://studentrepo.iium.edu.my/handle/123456789/9517
  record info:
    record content source: UIAM
    record creation date: 190725
    language of cataloging:
      language term: eng