rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Isolation and characterization of lactic acid bacteria isolated from blacktip shark (carcharhinus limbatus) with probiotic potential
  name:
    name part: Alotaibi, Mohammed Abdullah
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: Tengku Haziyamin Tengku Abdul Hamid
    role:
      role term: degree supervisor
  name:
    name part: Mohd Hamzah Mohd Nasir
    role:
      role term: degree supervisor
  name:
    name part: International Islamic University Malaysia
    name part: Department of Biotechnology
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2020
  language:
    language term: eng
  physical description:
    extent: xiv, 107 leaves : colour illustrations ; 30cm.
  abstract: A complex and diverse range of microorganism communities live within the gastrointestinal (GI) tract of living organisms. Amongst the bacterial communities in the GI tract, lactic acid bacteria (LAB) is generally regarded as safe, and probiotic due to their inherent benefits in improving the overall host GI tract, limiting intestinal infections, combatting pathogenic bacteria and controlling some types of cancer. In this study, 80 isolates were obtained from brain, epidermal organ, stomach and the end of intestinal tract of Blacktip shark (Carcharhinus limbatus). Preliminary inhibition test using spot-on-lawn assay was conducted to eliminate the isolate with no antagonistic activity against selected indicator strains. Out of these 80, only 4 isolates showed antagonistic activities against selected indicator strains in which they were selected for further biochemical tests such as Gram-staining, catalase, oxidase, and motility, the were found to be Gram positive coccoid shape, lactose fermentation positive, and negative to both oxidase and catalase tests. The isolates FA1, FA2, FA3 and FA4 demonstrated broad range of activity spectrum by inhibiting the Gram-positive indicator strains (Staphylococcus aureus and Bacillus cereus) and the Gram-negative indicator strains (Escherichia coli, Pseudomonas aeruginosa, salmonella typhimurium, Vibrio alginolyticus and Vibrio parahaemolyticus); with the highest inhibition zone recorded at 20 ± 0.1 mm on V. parahaemolyticus using agar well diffusion method as a confirmatory test. The isolates were chosen for further characterization using 16S rRNA PCR amplification. Each sequence was respectively deposited in GenBank (NCBI) with an accession number: MN975529 for FA1, MN982712 for FA2, MN982711 for FA3 and MN982710 for FA4. These isolates were identified to be belonged to member of the Lactococcus lactis group with 98% homology. The cell free supernatant (CFS) of the isolates was harvested for further tests. Treatment of the CFS wi…
  note: by Mohammed Abdullah Alotaibi
  note: Abstracts in English and Arabic.
  note: "A thesis submitted in fulfilment of the requirement for the degree of Master of Science (Biotechnology)." --On title page.
  note: Thesis (MSBTS)--International Islamic University Malaysia, 2020.
  note: Includes bibliographical references (leaves 71-94).
  subject:
    geographic code: a-my---
  identifier: http://studentrepo.iium.edu.my/handle/123456789/10375
  record info:
    record content source: UIAM
    record creation date: 210120
    language of cataloging:
      language term: eng