rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Anticariogenic activities of three groups of Malaysian seaweed extracts against oral cariogenic bacteria
  name:
    name part: Nurul Najwa binti Zamimi
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Department of Biotechnology
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2019
  language:
    language term: eng
  physical description:
    extent: xviii, 135 leaves : colour illustrations ; 30cm.
  abstract: Dental caries is still deliberated as one of major oral health problems worldwide. Certain types of seaweeds are reported to have antimicrobial properties. Although there were many researches about seaweed, only few of them focused on anticariogenic activities towards oral bacteria. Therefore, this study aimed to investigate the anticariogenic activities of Sargassum polycystum (S. polycystum), Caulerpa lentillifera (C. lentillifera) and Kappaphycus striatum (K. striatum) extracts against Streptococcus mutans (S. mutans) and Lactobacillus casei (L. casei).This study is divided into three phases. Phase I was to determine the best solvent in extracting the seaweeds via soxhlet apparatus and determination based on the zone of inhibition through disk diffusion method. Characterization of the seaweeds bioactive compound was done using phytochemical and Gas Chromatography Mass Spectrometry (GC-MS) analyses. Phase II was to evaluate the anticariogenic activities through determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) analyses, and to determine the synergistic effect of all three species of seaweeds extracts and antimicrobial agent (chlorhexidine) combinations using checkerboard titration method. Phase III was to investigate the mode of action of seaweed extracts on cell membrane of cariogenic bacteria. It was evaluated using method of cellular metabolites leakage at absorbance of 260 and 280 nm. All of the experimental procedures were repeated three times (n=3). Phase I revealed that all seaweed extracts that extracting using n-hexane having a higher zone of inhibition against S. mutans and L. casei when compared to 0.015% of chlorhexidine as a positive control (p<0.05). Phase II study found that the MIC and MBC values for all seaweed extracts against S. mutans and L. casei were in the range of 2.5, 10, 20 mg/mL, respectively. On the other hand, Fractional Inhibitory Concentration (FIC) indexes from the checkerboard titr…
  note: by Nurul Najwa binti Zamimi
  note: Thesis (MSBSC)--International Islamic University Malaysia, 2019.
  note: Includes bibliographical references (leaves 110-126).
  subject:
    geographic code: a-my---
  record info:
    record content source: UIAM
    record creation date: 191105
    language of cataloging:
      language term: eng