Field performance and molecular analysis of 14 vegetable soybean [Glycine max (L.) Merrill) accessions /
Breeders are still searching for the adapted accessions of vegetable soybean to be cultivated under Malaysian environment. Finding diverse accessions will assist in developing more adapted accessions. Therefore, a study was conducted to evaluate the field performance of 14 vegetable soybean accessio...
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| Format: | Thesis |
| Language: | English |
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| Online Access: | Click here to view 1st 24 pages of the thesis. Members can view fulltext at the specified PCs in the library. |
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| Summary: | Breeders are still searching for the adapted accessions of vegetable soybean to be cultivated under Malaysian environment. Finding diverse accessions will assist in developing more adapted accessions. Therefore, a study was conducted to evaluate the field performance of 14 vegetable soybean accessions introduced from Asian Vegetable Research and Development Center (AVRDC), Taiwan. The experiments were conducted using Three Factorial Randomized Complete Block Design (RCBD) with three replications at two different locations representing two different soil types. The locations were; the mineral soil of International Islamic University Malaysia (IIUM) Glasshouse experimental field and the beach ridges interspersed swales (BRIS) soil of Institute Oceanography and Maritime Studies Research Station (IRS) Kuantan, Pahang. The traits including pod yield, number of pods per plant, number seeds per plant, days to emergence, days to flowering, days to harvest and plant height were evaluated. The ultimate objective of the study is to identify potential accessions for planting under Malaysian climatical condition. In addition, genetic diversity assessment was done to study the ISSR polymorphism among the 14 vegetable soybean accessions. Lastly, the protein expression was analyzed to detect the differentially expressed proteins in selected vegetable soybean accessions grown at both locations. The result showed that IIUMSOY1 was the most adapted accession under both mineral and BRIS soils. Combined analysis of variance (ANOVA) showed that the location, accession and year effects had the significant differences for all the measured traits. The interactions between accession and location revealed significant differences for all measured traits except for days to emergence. The location also interacted significantly with the year for all traits. Pearson's correlation was run to determine the relationship between the yield and yield components among the 14 accessions. The strongest and positive relationship was detected between number of pods per plant and number of seeds per plant (0.971**) whereas the weakest relationship was observed between days to emergence with number of pods per plant (-0.251**). Pod yield also correlated positively with protein content at mineral soil. For the molecular analysis, the percentage of polymorphism was 42.50%, which was moderate diversity. Meanwhile, ISSR marker analysis also revealed five major clusters through a phylogenetic tree construction with the similarity coefficient values varying from 0.34 to 0.81. Genetic patterns obtained from ISSR analysis can be helpful to assist in the selection of materials for future breeding. The protein profiling detected 10 differentially expressed proteins in IIUMSOY1, IIUMSOY11 and IIUMSOY14 grown at both soils. The identified proteins were responsible for storage, flavonoid metabolism, protein modification, stress, carbohydrate metabolism and respiratory chain. Findings from this study could assist the soybean breeders in varietal development, crop improvement and breeding programme in Malaysia. |
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| Physical Description: | xxii, 232 leaves : colour illustrations ; 30cm. |
| Bibliography: | Includes bibliographical references (leaves 164-188). |