Extraction, characterisation and bioassay activities of allelochemicals from 11 allelopathic species /

This study established that allelopathic species from different plant groups of tree, fern, sedge and herbaceous differ greatly with respect to types and concentrations of allelochemical content. A total of 11 allelopathic species were evaluated for quantitative and qualitative phenolic compounds co...

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Main Author: Razanah binti Ramya @ Abd Rahim (Author)
Format: Thesis
Language:English
Subjects:
Online Access:http://studentrepo.iium.edu.my/handle/123456789/9570
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041 |a eng 
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050 1 0 |a  QK898.A43 
100 0 |a Razanah binti Ramya @ Abd Rahim,  |e author  |9 25984 
245 1 |a Extraction, characterisation and bioassay activities of allelochemicals from 11 allelopathic species /  |c by Razanah binti Ramya @ Abd Rahim 
264 1 |a Kuala Lumpur :  |b International Institute for Halal Research & Training, International Islamic University Malaysia,  |c 2019 
300 |a xxi, 201 leaves :  |b colour illustrations ;  |c 30cm. 
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500 |a Abstracts in English and Arabic. 
501 |a "A thesis submitted in fulfilment of the requirement for the degree of Doctor of Philosophy in Halal Industry." --On title page. 
502 |a Thesis (Ph.D)--International Islamic University Malaysia, 2019. 
504 |a Includes bibliographical references (leaves 157-172). 
520 |a This study established that allelopathic species from different plant groups of tree, fern, sedge and herbaceous differ greatly with respect to types and concentrations of allelochemical content. A total of 11 allelopathic species were evaluated for quantitative and qualitative phenolic compounds composition in different plant groups, extraction method, solvents and bioassay activities. The allelopathic effect towards weeds germination and growth were also tested by developing an in vitro model system. The main phenolic compounds identified in 11 allelopathic species were 4-Hydroxybenzoic acid, Caffeic acid, Vanillic acid, trans-p-Coumaric acid, Ferulic acid, 3-Coumaric acid and 2-Coumaric acid. The ratio of these phenolic compounds varies between plant groups. Tree group was found to have the highest total phenolic content followed by a group of ferns, herbaceous and sedges. 4-Hydroxybenzoic acid (3.00 to 1169.53 μg/g DW), Vanillic acid (1.88 to 1594.08 μg/g DW), trans-p-Coumaric acid (1.50 to 1171.28 μg/g DW), Ferulic acid (1.68 to 1876.0 μg/g DW), 3-Coumaric acid (15.58 to 933.63 μg/g DW) and 2-Coumaric acid (230.30 μg/g DW) were detected predominantly in tree group whereas Caffeic acid was found predominantly in herbaceous group (1.95 to 3375.23 μg/g DW). Marked differences were observed between the trees, ferns, herbaceous and sedges. Therefore, 11 allelopathic species were further analysed over bioassay activities to assess antimicrobial and weeds inhibitory. Antimicrobial activities of 11 allelopathic species in this study confirmed that the antifungal assay showed lower inhibitory than antibacterial assay. A. auriculiformis, M. cajuputi, D. linearis and H. malayana showed great antibacterial activity towards S. aureus, S. epidermis, E. coli, MRSA and P. aeruginosa whereas for antifungal only C. albicans and Fusarium sp were inhibited. A high-throughput in vitro model system for investigating allelopathic effect in weeds was developed and validated by assessing the allelopathic effects at a different concentration, a period of time and type of weed. The most influential factor appeared to be allelochemical extract concentration. The higher the concentration, the greater the inhibitory effect regardless type of weed. 
650 0 |a Allelochemical   |9 25985 
650 0 |a Allelopathic agents  |9 25986 
650 0 |a Allelopathy  |9 25987 
650 0 |a Plant extracts  |9 25988 
655 7 |a Theses, IIUM local 
690 |a Dissertations, Academic  |x International Institute for Halal Research & Training  |z IIUM  |9 8464 
710 2 |a International Islamic University Malaysia.  |b International Institute for Halal Research & Training 
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