rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: effects of activated virgin coconut oil (AVCO) and virgin coconut oil (VCO) on cell membrane of Candida albicans
  name:
    name part: Nor Izzah binti Mukhtar
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: International Islamic University Malaysia
    name part: Department of Biotechnology
  genre: Theses, IIUM local
  origin info:
    place:
      place term: my
    date issued: 2019
  language:
    language term: eng
  physical description:
    extent: xvi, 80 leaves ; illustrations ; 30cm.
  abstract: Virgin coconut oil (VCO) has been used in treating infections for ages. VCO can be used in preventing and protecting human body from microbial and fungal infections which can cause disease such as oral candidiasis. Oral candidiasis is an infection which is caused by the overgrowth of Candida albicans. However, the knowledge on the mechanism of action of AVCO and VCO on the cell membrane of C. albicans is limited. The aim of this study is to investigate the effects of activated virgin coconut oil (AVCO) and the crude extract of VCO on the cell membrane lysis and the components of cytoplasmic contents of Candida albicans. The differences of fatty acids composition in AVCO and VCO was analysed by using gas chromatography-mass spectrometry (GC-MS) method. To elucidate the effects of AVCO and VCO, live/dead bacterial viability kit was used to investigate the cell viability of Candida albicans in vitro cultures and was observed under fluorescence microscopy. Transmission electron microscopy was used to examine the cellular morphology of Candida albicans after being treated with AVCO and VCO. The cellular leakage of Candida albicans was detected by using UV/Vis spectrophotometer. Cytoplasmic leakage confirms creation of holes in the membrane by AVCO. Disturbance of cell membrane causes pore through which cellular materials (protein and DNA) leakage takes place. AVCO has been proven to have an antifungal effect on Candida albicans by altering the cell walls, disintegrating fungal cell membranes. The results of this study suggested that AVCO may disrupt the structure of the cell membrane and causes the release of cytoplasmic materials of Candida albicans due to the destruction of membrane network. Nevertheless, VCO has shown limited or no antifungal activity on the cell membrane of C. albicans. The study of cellular leakage of cell membrane components of Candida albicans may help to understand the mechanism of action of AVCO which has the potential to be a new antifungal tre…
  note: by Nor Izzah binti Mukhtar
  note: Abstracts in English and Arabic.
  note: "A thesis submitted in fulfilment of the requirement for the degree of Master of Science (Biosciences)." --On title page.
  note: Thesis (MSBSC)--International Islamic University Malaysia, 2019.
  note: Includes bibliographical references (leaves 63-75).
  subject:
    geographic code: a-my---
  subject:
    topic: Coconut oil
    topic: Therapeutic use
  subject:
    topic: Antifungal agents
  subject:
    topic: Candida albicans
  classification: QP144.O44
  identifier: https://lib.iium.edu.my/mom/services/mom/document/getFile/hU3Bf6lcDjuMYYKfKYYsL9J4LOi5c6Iq20200630151824976
  record info:
    record content source: UIAM
    record creation date: 190708
    language of cataloging:
      language term: eng