rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Effects of chronic low dose organic arsenic exposure on the liver of Sprague Dawley rats
  name:
    name part: Shahida Saharuddin
    role:
      role term: creator
    role:
      role term: author
  name:
    name part: Zunariah Buyong
    role:
      role term: degree supervisor
  name:
    name part: Norlelawati A. Talib
    role:
      role term: degree supervisor
  name:
    name part: Nor Zamzila Abdullah
    role:
      role term: degree supervisor
  name:
    name part: Jamalludin Ab. Rahman
    role:
      role term: degree supervisor
  name:
    name part: International Islamic University Malaysia
    name part: Kulliyyah of Medicine
  genre: Theses, IIUM local
  origin info:
    date issued: 2021
  language:
    language term: eng
  physical description:
    extent: xviii, 240 leaves : colour illustrations ; 30 cm.
  abstract: Monosodium methylarsonate (MSMA) is a potent organoarsenical herbicide that is still being used in most Asian countries, despite its restriction in some other countries. Organic arsenic has been given less attention as it thought to be less toxic than inorganic counterpart. In most studies, the reported adverse effects were mainly on gastrointestinal system with little information on its severity to the liver. The objective of this study was to investigate the effect of organic arsenic (MSMA) exposure on the liver. Sixty rats were divided into three groups with different duration of exposure. The rats were given MSMA at 63.20 mg/kg daily for 2, 4 and 6 months through oral gavage. Serum samples were analysed for AST, ALT and ALP. Arsenic accumulation measurement, histomorphometric evaluation (H&E, PAS, reticulin and TUNEL staining) and ultrastructural study (scanning and transmission electron microscopy) were done on liver tissue. LSEC were isolated for gene expression study. Accumulation of arsenic were significantly higher in the MSMA-exposed rats compared to their control with the highest in the 6-month group [2-month (3.97± 2.28, p=0.009), 4-month (4.57±0.47), p<0.001 and 6-month (21.33±9.83, p=0.004) µg/g]. Both ALT [Control: 85.3± 13.0, Exposed: 52.0±5.2, p=0.005] and ALP [Control: 237.6±52.8, Exposed: 162.9± 28.9, p=0.007] were significantly lower in 4-month MSMA-exposed group than their control.The difference in AST level in all groups were not significant. Histopathological and ultra-structurally, focal necrotic, apoptotic and fibrotic changes in the liver with the reduction of organelles in hepatocytes were observed in 4- and 6-month exposed rats. In 4-month exposed group, the liver displayed increased in ballooning degeneration of the hepatocytes at zone 2, focal necrosis with minimal inflammatory infiltrates with fibrosis (mixture of stage 1 and 2). Disrupted hepatic cords with hepatocytes blebs were seen. In 6-month exposed rats, more extensive changes w…
  note: by Shahida Saharuddin
  note: Abstracts in English and Arabic.
  note: "A thesis submitted in fulfilment of the requirement for the degree of Doctor of Philosophy in Medical Sciences." --On title page.
  note: Thesis (Ph.D)--International Islamic University Malaysia, 2021.
  note: Includes bibliographical references (leaves 204-231).
  subject:
    geographic code: a-my---
  subject:
    topic: Arsenic
    topic: Physiological effect
  subject:
    topic: Arsenic
    topic: Toxicology
  subject:
    topic: Liver cells
  classification: QP535.A7
  identifier: http://studentrepo.iium.edu.my/handle/123456789/10929
  record info:
    record content source: UIAM
    record creation date: 220411
    record change date: 20230621114728.0
    language of cataloging:
      language term: eng