The use of primary chondrocytes in combination with polycistronic sox-trio plasmid and tert gene transfer for articular cartilage tissue engineering and its bioethical implications /
A polycistronic SOX-trio plasmid vector was designed using VectorBuilder free software. The combination of SOX5, SOX6 and SOX9 genes resulted in a plasmid vector of more than ten (10) kbp. It is challenging to transfect primary cells culture with a larger than two (2) kbp DNA fragment. Thus, the SOX...
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| Format: | Thesis |
| Language: | English |
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| Online Access: | http://studentrepo.iium.edu.my/handle/123456789/11005 |
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