Dielectrophoresis To Manipulate Cells For Alzheimer's Research
The Alzheimer’s disease (AD) is one of the most common forms of dementia obliterates brain cells and thereafter, affects behavioral and physical aspect of the patient. Researchers put extensive and maximum efforts to eliminate or at least to reduce the progression of this wide dementia from getting...
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| Format: | Thesis |
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2021
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| Summary: | The Alzheimer’s disease (AD) is one of the most common forms of dementia obliterates brain cells and thereafter, affects behavioral and physical aspect of the patient. Researchers put extensive and maximum efforts to eliminate or at least to reduce the progression of this wide dementia from getting to advance level of its risk. They tried different approaches to fight and stand against AD. These approaches include pharmaceutical approach, electrical approach and so forth. In addition, it is disappointing that there is no unique and sophisticated solution to completely cure AD. In this project, the design of dielectrophoresis (DEP) platform is created as it may deform or dissolve amyloid-beta proteins (Aβ) that is the cause of AD. This research employed dielectrophoresis to a yeast cell suspension containing (Aβ) in a microfluidic environment. The Aβ was separated from the cells and characterized using the gradual dissolution of Aβ as a function of the applied DEP parameters. This research established the gradual dissolution of Aβ under specific DEP parameters. Further, Aβ in the fibril form at the tip of the electrode dissolved at high frequency. This was due to the conductivity of the suspending medium changing according to the frequency at 100MHz, that resulted in a higher temperature 32.925℃ at the tips of the electrodes, and consequently in the breakdown of the hydrogen bonds. However, those shaped as spheroidal monomers experienced a delay in the Aβ fibril transformation process. Yeast cells exposed to relatively low temperatures 0.01℃ at the base of the electrode that do not experience a positive or negative change in viability. The DEP microfluidic platform incorporating the integrated micro-tip electrodes array were able to selectively manipulate the yeast cells and dissolve the Aβ to a controlled extent. Suitable DEP parameters were demonstrated in term of voltage and frequency to induce such manipulation that is highly relevant for Aβ-related colloidal microfluidic research and could be applied to Alzheimer’s research. In the future, it can be by designing multifunctional Nano electrodes to act as sensor in order to attract the |
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