Molecular characterisation of multi-drug resistant isolates of Mycobacterium Tuberculosis in Malaysia
The rapid identification of resistance to primary anti-tuberculous agents is essential for the efficient treatment and control of multidrug resistant (MDR) Mycobacteria tuberculosis (MTB) strains. It is known that resistance to rifampicin (RIF) and isoniazid (INH) is a key factor in determining t...
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| Format: | Thesis |
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2012
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| Summary: | The rapid identification of resistance to primary anti-tuberculous agents is essential for the efficient treatment and control of multidrug resistant (MDR) Mycobacteria tuberculosis (MTB) strains. It is known that resistance to rifampicin (RIF) and isoniazid
(INH) is a key factor in determining the effectiveness of the currently recommended standard treatment regimens. In this study, three loci associated with drug resistance were selected for characterization; they were rpoB (RIF), katG and inhA (INH). A total of 129 sequential isolates of M. tuberculosis were found to be both RIF and INH resistant
(MDR) by conventional microbiological testing were screened for mutations associated with resistance to these drugs based on PCR amplification and followed by two molecular methods, sequence and restriction analysis. |
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