A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud

The aqueous crude root extract of Prismatomeris cf glabra was assessed for cytotoxic activity using tetrazolium compound (3-( 4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-( 4-sulfophenyl)-2H-tetrazolium, inner salt; MTS) and the electron coupling reagent, phenazine methosulfate (PMS) for...

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Main Author:	Mahmud, Omemah
Format:	Thesis
Language:	English
Published:	2007
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/100079/1/100079.PDF
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id	my-uitm-ir.100079
record_format	uketd_dc
spelling	my-uitm-ir.1000792024-08-26T05:06:48Z A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud 2007 Mahmud, Omemah RM Therapeutics. Pharmacology RS Pharmacy and materia medica The aqueous crude root extract of Prismatomeris cf glabra was assessed for cytotoxic activity using tetrazolium compound (3-( 4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-( 4-sulfophenyl)-2H-tetrazolium, inner salt; MTS) and the electron coupling reagent, phenazine methosulfate (PMS) for cellular viability. This study utilized two different types of human cancer cell lines, hepatocellular cancer, Hep G2 and leukemic cancer cell line, K562 cells, and the normal hepatocyte cell line, WRL 68. The results showed that the root extract of Prismatomeris cf glabra did not exhibit any cytotoxicity against all cell lines under study, with ICso value of 482.8 µg/ml for WRL 68, ICso value of 991.5 µg/ml for Hep G2 cell lines and ICso value of 2110.7 µg/ml for K562 cells after 24h incubation. These IC50 values were much larger the standard criterion established by the American National Cancer Institute (NCI). However, further experiments need to be conducted in order to confirm the results obtained in this preliminary study. 2007 Thesis https://ir.uitm.edu.my/id/eprint/100079/ https://ir.uitm.edu.my/id/eprint/100079/1/100079.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Sulong, Adnan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Sulong, Adnan
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Mahmud, Omemah A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud
description	The aqueous crude root extract of Prismatomeris cf glabra was assessed for cytotoxic activity using tetrazolium compound (3-( 4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-( 4-sulfophenyl)-2H-tetrazolium, inner salt; MTS) and the electron coupling reagent, phenazine methosulfate (PMS) for cellular viability. This study utilized two different types of human cancer cell lines, hepatocellular cancer, Hep G2 and leukemic cancer cell line, K562 cells, and the normal hepatocyte cell line, WRL 68. The results showed that the root extract of Prismatomeris cf glabra did not exhibit any cytotoxicity against all cell lines under study, with ICso value of 482.8 µg/ml for WRL 68, ICso value of 991.5 µg/ml for Hep G2 cell lines and ICso value of 2110.7 µg/ml for K562 cells after 24h incubation. These IC50 values were much larger the standard criterion established by the American National Cancer Institute (NCI). However, further experiments need to be conducted in order to confirm the results obtained in this preliminary study.
format	Thesis
qualification_level	Bachelor degree
author	Mahmud, Omemah
author_facet	Mahmud, Omemah
author_sort	Mahmud, Omemah
title	A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud
title_short	A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud
title_full	A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud
title_fullStr	A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud
title_full_unstemmed	A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud
title_sort	preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, wrl 68 and cancer cell line of hep g2 and k562 cells / omemah mahmud
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2007
url	https://ir.uitm.edu.my/id/eprint/100079/1/100079.PDF
_version_	1811769057409499136

A preliminary study of in vitro cytotoxicity of prismatomeris cf glabra against normal liver cell line, WRL 68 and cancer cell line of HEP G2 and K562 cells / Omemah Mahmud

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