Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin

Over the past decades male infertility cases have alarmingly increased among men worldwide. This issue calls for treatments. However, based on previous studies, existing treatments gave detrimental effects on male health especially on the male reproductive system itself. Thus, this study aims to dis...

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Main Author:	Sarpin, Nabihah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH Natural history - Biology
Online Access:	https://ir.uitm.edu.my/id/eprint/100210/1/100210.pdf
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id	my-uitm-ir.100210
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spelling	my-uitm-ir.1002102024-09-17T04:18:00Z Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin 2019 Sarpin, Nabihah QH Natural history - Biology Over the past decades male infertility cases have alarmingly increased among men worldwide. This issue calls for treatments. However, based on previous studies, existing treatments gave detrimental effects on male health especially on the male reproductive system itself. Thus, this study aims to discover new natural-based treatment that will enhance the reproductive parameters and at the same time gave insignificant side effects on the body system. This study was conducted to compare the effects of Cosmos caudatus, Piper sarmentosum and Premna cordifolia ethanolic extracts treated mice on haematological, biochemical, hormonal, reproductive parameters, selected organs histological structure and body weight changes. Twenty four sexually matured male mice were divided into 4 groups with 6 mice each. Group 1 (n=6) was given 0.9% saline solution (SS) and served as the control. Groups 2-4 (n=6, each group) were administered orally with 500 mg/kg bwt of CCEE, PSEE and PCEE respectively, for 30 days. The body weights were recorded before and after the treatments. MCHC of PSEE group was significantly higher than SS and CCEE groups but insignificant to PCEE group. In biochemical analysis, alanine aminotransferase (ALT) concentration of CCEE treated mice (45.13 ± 0.65 U/L) was significantly higher (p<0.05) than SS (27.85 ± 1.77 U/L) and PSEE (30.90 ± 2.86 U/L) groups. Meanwhile, aspartate aminotransferase (AST) concentration in PCEE group (128.73 ± 10.91 U/L) was significantly higher (p<0.05) than SS group (57.68 ± 3.36 U/L). The total protein of CCEE treated mice (71.00 ± 3.06 g/L) was significantly higher (p<0.05) than SS (54.55 ± 1.21 g/L) and PSEE (56.30 ± 0.86 g/L) groups but not to PCEE group (64.20 ± 3.46 g/L). Testosterone level of PSEE treated mice (4.47±0.87 ng/ml) was significantly higher (p<0.05) in comparison with SS (2.12±0.71 ng/ml), CCEE group (1.77±0.14 ng/ml) and PCEE (2.32±0.21 ng/ml). Sperm motility of mice treated with PSEE (47.17 ±5.93%) significantly increased (p<0.05) compared to only SS group (16.60± 4.26%). PCEE treated mice significantly decreased (p<0.05) the percentage of normal sperm morphology (70.80 ± 3.76%) compared to SS group (92.78 ± 2.51%). PCEE group showed significantly the highest score (9.43 ±0.04) in Johnsen scoring when compared to SS (8.98±0.05) and CCEE (9.29±0.04) groups. Both testis and cauda epididymis tissue sections from all groups showed normal morphology. However, liver and kidney of CCEE and PCEE treated mice showed minor histological changes. In conclusion, PSEE has the potential in enhancing sperm parameters with insignificant side effects to the studied organs. 2019 Thesis https://ir.uitm.edu.my/id/eprint/100210/ https://ir.uitm.edu.my/id/eprint/100210/1/100210.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	QH Natural history - Biology
spellingShingle	QH Natural history - Biology Sarpin, Nabihah Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin
description	Over the past decades male infertility cases have alarmingly increased among men worldwide. This issue calls for treatments. However, based on previous studies, existing treatments gave detrimental effects on male health especially on the male reproductive system itself. Thus, this study aims to discover new natural-based treatment that will enhance the reproductive parameters and at the same time gave insignificant side effects on the body system. This study was conducted to compare the effects of Cosmos caudatus, Piper sarmentosum and Premna cordifolia ethanolic extracts treated mice on haematological, biochemical, hormonal, reproductive parameters, selected organs histological structure and body weight changes. Twenty four sexually matured male mice were divided into 4 groups with 6 mice each. Group 1 (n=6) was given 0.9% saline solution (SS) and served as the control. Groups 2-4 (n=6, each group) were administered orally with 500 mg/kg bwt of CCEE, PSEE and PCEE respectively, for 30 days. The body weights were recorded before and after the treatments. MCHC of PSEE group was significantly higher than SS and CCEE groups but insignificant to PCEE group. In biochemical analysis, alanine aminotransferase (ALT) concentration of CCEE treated mice (45.13 ± 0.65 U/L) was significantly higher (p<0.05) than SS (27.85 ± 1.77 U/L) and PSEE (30.90 ± 2.86 U/L) groups. Meanwhile, aspartate aminotransferase (AST) concentration in PCEE group (128.73 ± 10.91 U/L) was significantly higher (p<0.05) than SS group (57.68 ± 3.36 U/L). The total protein of CCEE treated mice (71.00 ± 3.06 g/L) was significantly higher (p<0.05) than SS (54.55 ± 1.21 g/L) and PSEE (56.30 ± 0.86 g/L) groups but not to PCEE group (64.20 ± 3.46 g/L). Testosterone level of PSEE treated mice (4.47±0.87 ng/ml) was significantly higher (p<0.05) in comparison with SS (2.12±0.71 ng/ml), CCEE group (1.77±0.14 ng/ml) and PCEE (2.32±0.21 ng/ml). Sperm motility of mice treated with PSEE (47.17 ±5.93%) significantly increased (p<0.05) compared to only SS group (16.60± 4.26%). PCEE treated mice significantly decreased (p<0.05) the percentage of normal sperm morphology (70.80 ± 3.76%) compared to SS group (92.78 ± 2.51%). PCEE group showed significantly the highest score (9.43 ±0.04) in Johnsen scoring when compared to SS (8.98±0.05) and CCEE (9.29±0.04) groups. Both testis and cauda epididymis tissue sections from all groups showed normal morphology. However, liver and kidney of CCEE and PCEE treated mice showed minor histological changes. In conclusion, PSEE has the potential in enhancing sperm parameters with insignificant side effects to the studied organs.
format	Thesis
qualification_level	Master's degree
author	Sarpin, Nabihah
author_facet	Sarpin, Nabihah
author_sort	Sarpin, Nabihah
title	Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin
title_short	Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin
title_full	Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin
title_fullStr	Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin
title_full_unstemmed	Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin
title_sort	reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / nabihah sarpin
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/100210/1/100210.pdf
_version_	1811769063783792640

Reproductive, biochemical and haematological parameters of male mice treated with cosmos caudatus, piper sarmentosum and premna cordifolia ethanolic extract / Nabihah Sarpin

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