Antioxidant activity of the fruits of ficus deltoidea var angustifolia sp. (mas cotek) / Sulnalti Mustafa

This study was aimed at detecting the presence of alkaloids, flavonoids and terpenes and also to compare the antioxidant activities of hexane, chloroform and methanol extracts of sample from fruits of Ficus deltoidea (var angustifolia sp.). This experiment used successive extraction method for the e...

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Bibliographic Details
Main Author: Mustafa, Sulnalti
Format: Thesis
Language:English
Published: 2008
Online Access:https://ir.uitm.edu.my/id/eprint/100851/1/100851.pdf
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Summary:This study was aimed at detecting the presence of alkaloids, flavonoids and terpenes and also to compare the antioxidant activities of hexane, chloroform and methanol extracts of sample from fruits of Ficus deltoidea (var angustifolia sp.). This experiment used successive extraction method for the extraction process using three solvents of different polarity (hexane, chloroform and methanol). The antioxidant activity was determined by four methods; Total Phenolic Content (TPC) method, Ferric Thiocyanate (FTC) method, Thiobarbituric Acid (TBA) method while the radical scavenging activity was measured by the 2,2-diphenyl-1-picryhydrazyl (DPPH) method. All tested extracts exhibited very strong antioxidant potential. The hexane extract showed the highest percent inhibition followed by methanol extract and chloroform extract as compared to quercetin and vitamin E. In the FTC method, the hexane extract showed the strongest antioxidant activity (97.78%) followed by methanol extract (96.69%) and chloroform extract (93.53%). In the TBA assay, the hexane extract showed the strongest antioxidant activity (95.58%) followed by methanol extract (91.40%) and chloroform extract (90.70%). In the DPPH method, the chloroform extract from fruits of Ficus deltoidea (var angustifolia sp.) exhibited the strongest radical scavenging activity (103.83%) followed by methanol extract (95.90%) as compared to quercitin as reference standard. A similar trend was observed for total phenolic content (TPC) where the hexane extract of fruits of Ficus deltoidea (var angustifolia sp.) showed the greatest total phenolic content (12.96 ppm) followed by methanol extract (12.26 ppm) and chloroform extract (7.96 ppm).