Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan

Natural products have played an important role in treating cancer by serving as compounds in their natural form or as templates for synthetic compounds. In the recent years, endophytes hold a new source of novel cytotoxic drug discovery. The endophyte HAB10R12 used throughout this study was isolated...

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Main Author:	Roslan, Nor Ashiqin Aiman
Format:	Thesis
Language:	English
Published:	2008
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/100879/1/100879.PDF
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id	my-uitm-ir.100879
record_format	uketd_dc
spelling	my-uitm-ir.1008792024-08-26T04:59:50Z Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan 2008 Roslan, Nor Ashiqin Aiman RM Therapeutics. Pharmacology RS Pharmacy and materia medica Natural products have played an important role in treating cancer by serving as compounds in their natural form or as templates for synthetic compounds. In the recent years, endophytes hold a new source of novel cytotoxic drug discovery. The endophyte HAB10R12 used throughout this study was isolated from a local medicinal plant. Preliminary screening found this endophyte to possess cytotoxic activity against P388, a murine leukaemic cell line. The aim of this study was to determine the effect of different culture conditions on the cytotoxic metabolite production of HAB I OR 12 against human colon (HCTl 16) and breast (MCF7) cancer cell lines. The various fermentation parameters include type of media (agar vs. broth), water source (tap water vs. deionized water), incubation time ( 1, 2 and 4 weeks) as well as strength of media ( full media strength vs. one fifth media strength). The sensitivity of HAB10R12 extracts encoded PDAl, PDA2, PDA4, PDB2(B), PDB2(M), TPDA, TPDB(B), TPDB(M), l/5PDA, l/5PDB(B) and l/5PDB(M) were determined against HCTI 16 and MCF7 cell lines. All the extracts were evaluated for in vitro cytotoxic activity against HCTI 16 and MCF7 cell lines using the MTT assay. The sensitivity of each cell line was characterized by the IC50 value. The result of this study indicated that endophytic fungus HAB10R12 incubated for 4 weeks and that prepared by using full strength medium with deionized water to possess stronger cytotoxic activity when compared to other fermentation parameters. The endophytic fungus HAB10R12 with its promising cytotoxic activity has the potential to be developed as cytotoxic drug. 2008 Thesis https://ir.uitm.edu.my/id/eprint/100879/ https://ir.uitm.edu.my/id/eprint/100879/1/100879.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ramasamy, Kalavathy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ramasamy, Kalavathy
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Roslan, Nor Ashiqin Aiman Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan
description	Natural products have played an important role in treating cancer by serving as compounds in their natural form or as templates for synthetic compounds. In the recent years, endophytes hold a new source of novel cytotoxic drug discovery. The endophyte HAB10R12 used throughout this study was isolated from a local medicinal plant. Preliminary screening found this endophyte to possess cytotoxic activity against P388, a murine leukaemic cell line. The aim of this study was to determine the effect of different culture conditions on the cytotoxic metabolite production of HAB I OR 12 against human colon (HCTl 16) and breast (MCF7) cancer cell lines. The various fermentation parameters include type of media (agar vs. broth), water source (tap water vs. deionized water), incubation time ( 1, 2 and 4 weeks) as well as strength of media ( full media strength vs. one fifth media strength). The sensitivity of HAB10R12 extracts encoded PDAl, PDA2, PDA4, PDB2(B), PDB2(M), TPDA, TPDB(B), TPDB(M), l/5PDA, l/5PDB(B) and l/5PDB(M) were determined against HCTI 16 and MCF7 cell lines. All the extracts were evaluated for in vitro cytotoxic activity against HCTI 16 and MCF7 cell lines using the MTT assay. The sensitivity of each cell line was characterized by the IC50 value. The result of this study indicated that endophytic fungus HAB10R12 incubated for 4 weeks and that prepared by using full strength medium with deionized water to possess stronger cytotoxic activity when compared to other fermentation parameters. The endophytic fungus HAB10R12 with its promising cytotoxic activity has the potential to be developed as cytotoxic drug.
format	Thesis
qualification_level	Bachelor degree
author	Roslan, Nor Ashiqin Aiman
author_facet	Roslan, Nor Ashiqin Aiman
author_sort	Roslan, Nor Ashiqin Aiman
title	Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan
title_short	Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan
title_full	Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan
title_fullStr	Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan
title_full_unstemmed	Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan
title_sort	enhancement of cytotoxic metabolite production from hab10r12 an endophyte / nor ashiqin aiman roslan
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/100879/1/100879.PDF
_version_	1811769102836957184

Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan

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