Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin
Biotransformation has been a subject of interest since centuries ago. It can be used to modify a compound into another compound, which may be hard to obtain using chemical synthesis, by using microorganisms such as endophytes. The study is aimed to carry out the fermentation of a given substrate, to...
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2008
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my-uitm-ir.1008902024-08-26T04:58:20Z Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin 2008 Mohd Yasin, Syuhaida QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica Biotransformation has been a subject of interest since centuries ago. It can be used to modify a compound into another compound, which may be hard to obtain using chemical synthesis, by using microorganisms such as endophytes. The study is aimed to carry out the fermentation of a given substrate, to identify the biotransformation product, as well as to rationalize the chemical modification induced by the endophytes and compare with standard organic chemistry procedure. It was done by culturing endophytes, feeding them with substrates, incubating the culture for one to two weeks, filtering and extracting the mycelium, and lastly analyzing the extract by using Thin Layer Chromatography, Nuclear Magnetic Resonance, and High Performance Liquid Chromatography technique. It was found that biotransformation of (+)-a-longipinene by Burkholderia has taken place in the experiment. In conclusion, the biotransformation of substrate had been successfully done but further study should be done to purify and identify the resulting compound since it could not be done in this study due to time-constraint. 2008 Thesis https://ir.uitm.edu.my/id/eprint/100890/ https://ir.uitm.edu.my/id/eprint/100890/1/100890.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Abdullah, Jean-Frederic Faizal Weber |
| institution |
Universiti Teknologi MARA |
| collection |
UiTM Institutional Repository |
| language |
English |
| advisor |
Abdullah, Jean-Frederic Faizal Weber |
| topic |
QD Chemistry QD Chemistry RS Pharmacy and materia medica |
| spellingShingle |
QD Chemistry QD Chemistry RS Pharmacy and materia medica Mohd Yasin, Syuhaida Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin |
| description |
Biotransformation has been a subject of interest since centuries ago. It can be used to modify a compound into another compound, which may be hard to obtain using chemical synthesis, by using microorganisms such as endophytes. The study is aimed to carry out the fermentation of a given substrate, to identify the biotransformation product, as well as to rationalize the chemical modification induced by the endophytes and compare with standard organic chemistry procedure. It was done by culturing endophytes, feeding them with substrates, incubating the culture for one to two weeks, filtering and extracting the mycelium, and lastly analyzing the extract by using Thin Layer Chromatography, Nuclear Magnetic Resonance, and High Performance Liquid Chromatography technique. It was found that biotransformation of (+)-a-longipinene by Burkholderia has taken place in the experiment. In conclusion, the biotransformation of substrate had been successfully done but further study should be done to purify and identify the resulting compound since it could not be done in this study due to time-constraint. |
| format |
Thesis |
| qualification_level |
Bachelor degree |
| author |
Mohd Yasin, Syuhaida |
| author_facet |
Mohd Yasin, Syuhaida |
| author_sort |
Mohd Yasin, Syuhaida |
| title |
Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin |
| title_short |
Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin |
| title_full |
Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin |
| title_fullStr |
Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin |
| title_full_unstemmed |
Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin |
| title_sort |
malaysian endophytes as a tool of chemical modification / syuhaida mohd yasin |
| granting_institution |
Universiti Teknologi MARA (Kampus Puncak Alam) |
| granting_department |
Faculty of Pharmacy |
| publishDate |
2008 |
| url |
https://ir.uitm.edu.my/id/eprint/100890/1/100890.PDF |
| _version_ |
1811769105357733888 |