Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad

An endophyte HAB2 I F23 used in this study was grown in various culture conditions and the extracts were tested for antimicrobial and cytotoxic activity. The parameters that were used in this study include different form (solid, liquid) of potato dextrose media at different incubation period (one, t...

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Main Author:	Mohamad, Munirah
Format:	Thesis
Language:	English
Published:	2008
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/100915/1/100915.PDF
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id	my-uitm-ir.100915
record_format	uketd_dc
spelling	my-uitm-ir.1009152024-08-26T04:38:16Z Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad 2008 Mohamad, Munirah RM Therapeutics. Pharmacology RS Pharmacy and materia medica An endophyte HAB2 I F23 used in this study was grown in various culture conditions and the extracts were tested for antimicrobial and cytotoxic activity. The parameters that were used in this study include different form (solid, liquid) of potato dextrose media at different incubation period (one, two and four weeks), water source (tap water, deionized water) of solid and liquid potato dextrose media and 1/5 diluted solid and liquid potato dextrose media. Extracts of HAB21 F23 grown in various culture conditions exhibited considerable differences in cytotoxic activity when tested in vitro against colon cancer (HT29) cell lines and normal embryonic liver (WRL68) cell lines using the MTT assays. The results from the present study showed that among the solid media, PDA2 was the most potent with an IC50 value of 3.57 µg/ml and among the liquid media, l/5PDB was the most potent towards HT29 cell lines with an IC50 value of 0.43 µg/ml. However, the HAB2 I F23 extract was potent also against the cell lines WRL68, showing that it was not selective against cancer and normal cell lines. The HAB21 F23 extracts were also tested for antimicrobial activity against pathogenic fungi (Candida albicans, Aspergillus niger), and bacteria (Staphylococcus aureus, Bacillus subtilis, E. coli, Micrococcus luteus) using disc diffusion method but results from this study revealed that the endophyte HAB21 F23 showed no antimicrobial activity. 2008 Thesis https://ir.uitm.edu.my/id/eprint/100915/ https://ir.uitm.edu.my/id/eprint/100915/1/100915.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ramasamy, Kalavathy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ramasamy, Kalavathy
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Mohamad, Munirah Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad
description	An endophyte HAB2 I F23 used in this study was grown in various culture conditions and the extracts were tested for antimicrobial and cytotoxic activity. The parameters that were used in this study include different form (solid, liquid) of potato dextrose media at different incubation period (one, two and four weeks), water source (tap water, deionized water) of solid and liquid potato dextrose media and 1/5 diluted solid and liquid potato dextrose media. Extracts of HAB21 F23 grown in various culture conditions exhibited considerable differences in cytotoxic activity when tested in vitro against colon cancer (HT29) cell lines and normal embryonic liver (WRL68) cell lines using the MTT assays. The results from the present study showed that among the solid media, PDA2 was the most potent with an IC50 value of 3.57 µg/ml and among the liquid media, l/5PDB was the most potent towards HT29 cell lines with an IC50 value of 0.43 µg/ml. However, the HAB2 I F23 extract was potent also against the cell lines WRL68, showing that it was not selective against cancer and normal cell lines. The HAB21 F23 extracts were also tested for antimicrobial activity against pathogenic fungi (Candida albicans, Aspergillus niger), and bacteria (Staphylococcus aureus, Bacillus subtilis, E. coli, Micrococcus luteus) using disc diffusion method but results from this study revealed that the endophyte HAB21 F23 showed no antimicrobial activity.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad, Munirah
author_facet	Mohamad, Munirah
author_sort	Mohamad, Munirah
title	Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad
title_short	Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad
title_full	Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad
title_fullStr	Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad
title_full_unstemmed	Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad
title_sort	enhancement of metabolite production from hab21 f23 an endophyte / munirah mohamad
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/100915/1/100915.PDF
_version_	1811769110617391104

Enhancement of metabolite production from HAB21 F23 an endophyte / Munirah Mohamad

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