Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor

The effect of culture condition on antimicrobial activity of endophytes HAB 11 R3 and HAB 1 OR 12 were investigated. The parameter that were investigated are incubation period (PDAl; incubated for I week, PDA2; incubated for 2 weeks, PDA4; incubated for 4 weeks), different media (solid; PDB2 (incuba...

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Main Author:	Md Nor, Siti Nadia Adilah
Format:	Thesis
Language:	English
Published:	2008
Subjects:	RM Therapeutics Pharmacology RS Pharmacy and materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/100917/1/100917.PDF
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id	my-uitm-ir.100917
record_format	uketd_dc
spelling	my-uitm-ir.1009172024-08-26T04:37:22Z Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor 2008 Md Nor, Siti Nadia Adilah RM Therapeutics. Pharmacology RS Pharmacy and materia medica The effect of culture condition on antimicrobial activity of endophytes HAB 11 R3 and HAB 1 OR 12 were investigated. The parameter that were investigated are incubation period (PDAl; incubated for I week, PDA2; incubated for 2 weeks, PDA4; incubated for 4 weeks), different media (solid; PDB2 (incubated in broth media), liquid; PDA2 (incubated in agar media)), different water source (tap water; TPDA and TPDB, deionized water; PDA2 and PDB2) and different strength (1/5 from original strength; 1/5PDA and l/5PDB). Two different methods were employed to detect the antimicrobial activity; the disc diffusion test and minimum inhibitory concentration (MIC) test. The secondary metabolites of HAB 11 R3 were showed killing activity towards one or more pathogenic bacteria. Endophytic metabolites that were cultured in broth media showed inhibitory activity against Bacillus subtilis (35mm) and Micrococcus luteus (38mm). The metabolites cultured in broth media also give the lowest minimum inhibitory concentration (MIC) against Bacillus subtilis (0.08mg/ml), Micrococcus luteus (0.04mg/ml) and Escherichia coli (0.63mg/ml) whereas the mycelium of endophyte that cultured in broth media give lowest MIC against Staphylococcus aureus (0.3 lmg/ml). No activity of antimicrobial activity was reported on endophytic strain HAB10R12. 2008 Thesis https://ir.uitm.edu.my/id/eprint/100917/ https://ir.uitm.edu.my/id/eprint/100917/1/100917.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Ramasamy, Kalavathy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ramasamy, Kalavathy
topic	RM Therapeutics Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics Pharmacology RS Pharmacy and materia medica Md Nor, Siti Nadia Adilah Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor
description	The effect of culture condition on antimicrobial activity of endophytes HAB 11 R3 and HAB 1 OR 12 were investigated. The parameter that were investigated are incubation period (PDAl; incubated for I week, PDA2; incubated for 2 weeks, PDA4; incubated for 4 weeks), different media (solid; PDB2 (incubated in broth media), liquid; PDA2 (incubated in agar media)), different water source (tap water; TPDA and TPDB, deionized water; PDA2 and PDB2) and different strength (1/5 from original strength; 1/5PDA and l/5PDB). Two different methods were employed to detect the antimicrobial activity; the disc diffusion test and minimum inhibitory concentration (MIC) test. The secondary metabolites of HAB 11 R3 were showed killing activity towards one or more pathogenic bacteria. Endophytic metabolites that were cultured in broth media showed inhibitory activity against Bacillus subtilis (35mm) and Micrococcus luteus (38mm). The metabolites cultured in broth media also give the lowest minimum inhibitory concentration (MIC) against Bacillus subtilis (0.08mg/ml), Micrococcus luteus (0.04mg/ml) and Escherichia coli (0.63mg/ml) whereas the mycelium of endophyte that cultured in broth media give lowest MIC against Staphylococcus aureus (0.3 lmg/ml). No activity of antimicrobial activity was reported on endophytic strain HAB10R12.
format	Thesis
qualification_level	Bachelor degree
author	Md Nor, Siti Nadia Adilah
author_facet	Md Nor, Siti Nadia Adilah
author_sort	Md Nor, Siti Nadia Adilah
title	Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor
title_short	Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor
title_full	Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor
title_fullStr	Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor
title_full_unstemmed	Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor
title_sort	enhancement of antimicrobial activity in endophytes hab11r3 and hab10r12 / siti nadia adilah md nor
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/100917/1/100917.PDF
_version_	1811769111117561856

Enhancement of antimicrobial activity in endophytes HAB11R3 and HAB10R12 / Siti Nadia Adilah Md Nor

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